1.11.1.14 isozyme H8 and W171 mutant 394606 1.11.1.14 purified enzyme, hanging drop vapor diffusion method, mixing of 0.001 ml of 8 mg/ml protein solution in 10 mM succinate buffer, pH 6.0, with 0.001 ml of reservoir solution containing 16% PEG 6000, and equilibration of the mixture against 0.5 ml of reservoir solution, 7 days at 20°C, X-ray diffraction structure determination and analysis at 1.67 A resolution, modeling 764351