5.1.99.7 purified recombinant enzyme, vapour diffusion method, mixing of 500 nl of 6 mg/ml protein in 20 mM Tris pH 7.5, 50 mM NaCl, with 500 nl of reservoir solution containing 40% v/v 1,2-propanediol, 100 mM HEPES, pH 7.5, 1 week, at room temperature, X-ray diffraction structure determination and analysis at 3.0 A resolution 733859