5.1.99.1 at 2.0 A resolutionm, crystals of recombinant enzyme are grown at 20°C using the hanging-drop, vapor-diffusion method, drops consisted of 2 microl of protein solution and 2 microl of mother liquor (0.1 M citric acid, pH 2.6, 1.6 M (NH4)2SO4), crystals are obtained after 7-8 days growth, native and derivative crystals are soaked in 2 M Li2SO4 706660 5.1.99.1 crystallization of native, Co2+-substituted and SeMet-substituted enzyme by hanging-drop vapour-diffusion method 653930 5.1.99.1 vapour diffusion method 649165