2.4.1.90 - 489546 2.4.1.90 catalytic enzyme domain without complexed substrate, conf-I' conformation, X-ray diffraction structure determination and analysis at 1.9 A resolution 659700 2.4.1.90 crystal structure of enzyme-alpha-lactalbumin complex with UDP-Glc 489538 2.4.1.90 crystal structure of the catalytic domain of beta4Gal-T7 from Drosophila melanogaster in the presence of manganese and UDP at 1.81 A resolution is shown. In the crystal structure, a new manganese ion-binding motif is observed. Superposition of the crystal structures of beta4Gal-T7 and beta4Gal-T1 shows that the catalytic pocket and the substrate-binding sites in these proteins are similar 719863 2.4.1.90 crystal structures of the beta4galactosyltransferase catalytic domain and its complex with uridine diphosphogalactose 489550 2.4.1.90 enzyme in complex with Mn2+, UDP-GalNAc, glucose, and alpha-lactalbumin, X-ray diffraction structure determination and analysis at 2.0 A resolution 658502 2.4.1.90 hanging drop vapor diffusion method, using 0.2 M potassium nitrate, 20% (w/v) PEG 3350 758173 2.4.1.90 M344H mutant enzyme complexed with UDP-Gal and Mn2+ or Mg2+, hanging drop vapour diffusion method, 30 mg/ml protein in solution with 17 mM UDP-Gal, and 17 mM MgCl2 or MnCl2, precipitated by 10% v/v dioxane, 100 mM MES-NaOH, pH 6.5, and 2.0M ammonium sulfate, room temperature, X-ray diffraction structure determination and analysis at 2.3 A resolution, determination of conformation stereochemistry, modeling 658032 2.4.1.90 mutant enzyme C342T/M344H, hanging drop vapor diffusion method, using 100 mM MES-NaOH buffer pH 6.5, 1.8 M ammonium sulfate and 10% (v/v) 1, 4-dioxane 736208 2.4.1.90 recombinant enzyme, crystal structure of lactose synthase reveals a large conformational change in its catalytic component, the beta1,4-galactosyltransferase-I 488948