2.3.1.B41 analysis of in silico cocrystal structure of SIRT6 bound to ADP-ribose and a H3K9 myristoylated peptide, PDB ID 3ZG6 756879 2.3.1.B41 crystals are grown by hanging drop method, structures of the enzyme bound to nictotinamide are determined from a single crystal diffracting to 2.4 A resolution. The structures show that free nicotinamide binds in a conserved pocket that participates in NAD+ binding and catalysis 730391 2.3.1.B41 hanging drop vapor diffusion at 20°C. Crystal structures in complex with ADP-ribose and the non-hydrolyzable analog of O-acetyl-ADP-ribose, 2'-N-acetyl-ADP-ribose 729972 2.3.1.B41 hanging drop vapor diffusion method at 18°C, crystal structure of Sirt6 in complex with a histone H3 K9 myristoyl peptide and ADPribose at 2.2 A resolution 730464 2.3.1.B41 hanging drop vapor diffusion method, cocrystal of enzyme with [histone H3 peptide]-N6-myristoyl-L-lysine 728852 2.3.1.B41 purified recombinant Sirt6 in complex with UBCS compounds, hanging drop vapour diffusion method, mixing of 10 mg/ml Sirt6-13-308, and 10 mM ADP-ribose at 20°C in with reservoir solution comtaining 1.6 M (NH4)2SO4, 10% PEG 400, and Bis-Tris, pH 5.7, crystals appear within 24 h and are subsequently soaked by adding 100 mM UBCS compound, incubation for one week, X-ray diffraction structure determination and analysis at 1.87-2.10 A resolution, molecular replacement using the protein part of a Sirt6/ADP-ribose structure (PDB code 3K35) as a search model, modeling 755787 2.3.1.B41 Sirt6/ADP-ribose/trichostatin A complex, hanging drop vapor diffusion method, 10 mg/ml purified recombinant protein Sirt6 and 10 mM ADP-ribose in 50 mM Na-phosphate buffer, pH 7.5, 0.6 mM DTT, and 5% DMSO are mixed with an equal volume of reservoir solution containing 1.6 M (NH4)2SO4, 10% PEG 400, and Bis-Tris buffer, pH 5.7, at 20°C, X-ray diffraction structure determination and analysis at 1.70 A resolution, molecular replacement using a Sirt6/ADP-ribose structure (PDB ID 3K35) as search model, modeling 757398 2.3.1.B41 vapor diffusion at 20°C, crystal structure of the enzyme bound to KGLGKGGA(N6-myristoyl)KRHRKW 730900