1.1.1.379 purified enzyme in complex with substrates NADH and anilino(oxo)acetate (AOA), hanging-drop vapor diffusion method, mixing of 10 mg/ml protein in 5 mM sodium MOPS, pH 7.5, 2 mM NADH, and 20 mM AOA with reservoir solution containing 0.1 M cacodylate-Na, pH 5.0, 0.17 M ammonium sulfate, and 22.5% PEG 8000, in a 1:1 ratio, at 25°C, X-ray diffraction structure determination and analysis at 2.4 A resolution, molecular replacement using the crystal structure of unliganded D-ManDH2 (PDB ID 3WFI) as a search model, modeling. The ternary complex of D-ManDH2 forms a 2fold symmetric homodimer in a crystallographic asymmetric unit, as in the cases of the apo and binary complex structures 762694 1.1.1.379 the enzyme is crystallized in three different forms using the hanging drop vapour diffusion method at 15-20°C. Type I crystals belong to space group P222(1), P22(1)2(1) or P2(1)2(1)2(1) with a = 100.3 A, b = 117.4 A, c = 80.4 A and are likely to contain a dimer in the crystallographic asymmetric unit 730201