6.2.1.64 APPBP1-UBA3 complex bound to NEDD8 mutant A72Q, hanging drop vapor diffusion method, using 0.1 M Tris, 0.4 M ammonium acetate, 9-10% (w/v) PEG 10K, 5 mM dithiothreitol, pH 7.0-8.0 6.2.1.64 quaternary complex between human APPBP1-UBA3, a heterodimeric E1, NEDD8 and ATP, hanging drop vapor diffusion method, using 10.5-10.7% (w/v) PEG 10k, 0.1 M Tris, 0.4 M NaCl, 10% (w/v) PEG400, 5 mM dithiothreitol (pH 8.0-8.5)