6.2.1.4 hanging drop vapour diffusion method using using 10% polyethylene glycol 3350, 100 mM 4-morpholinoethanesulfonic acid (pH 6.4 for 1 M solution) and 200 mM KCl and a protein solution containing 5 mM GDP and 10 mM MnCl2 671128 6.2.1.4 hanging-drop vapour diffusion method using a precipitant solution containing 10% (v/v) isopropanol, 100 mM ammonium sulfate, 20% (w/v) polyethylene glycol 4000, and 100 mM HEPES or Bicine, pH 7.5 674529 6.2.1.4 in complex with CoA, hanging drop vapor diffusion method, using 1.6 M ammonium sulfate, 1 mM 2-mercaptoethanol, 50 mM sodium/potassium phosphate pH 7.0 743957 6.2.1.4 in complex with succinate, magnesium ions and CoA, hanging drop vapor diffusion method, using 20% (w/v) polyethylene glycol 3350 and 0.2 M magnesium formate 743947 6.2.1.4 purified enzyme in complex with GDP-Mn2+, protein in 50 mM KCl, 0.1 mM EDTA, 50 mM Tris-HCl, pH 7.4, X-ray diffraction structure determination and analysis at 2.35 A resolution 726583