3.4.22.54 hanging-drop vapor diffusion method at 22°C, structures of calpain-3 Protease core mutant-C129S, calpain-3 protease core in complex with E-64, calpain-3 protease core in complex with leupeptin 3.4.22.54 model of calpain 3 by crystal structures of human calpain2 and calpastatin-inhibited rat calpain2