3.2.1.97 purified recombinant His-tagged enzyme, X-ray diffraction structure analysis at 2.0-2.5 A resolution 3.2.1.97 purified recombinant isolated catalytic domain, hanging drop vapour diffusion method at 19°C, 1 ml of 15 mg/ml protein in 20 mM Tris-HCl, pH 8.0, is mixed with 1 ml 25% PEG 1500. Removal of the His6-tag is unnecessary for crystallization. Crystals are cryoprotected in 1 ml 33% PEG 1500 supplemented with 6% MPD, X-ray diffraction structure determination and analysis at 2.0 A resolution