3.2.1.177 crystallographic structures of both the apo enzyme and the trapped covalent 5-fluoro-beta-xylosyl-enzyme intermediate, together with docking studies with the XXXG heptasaccharide the importance of a PA14 domain insert in the recognition of longer oligosaccharides by extension of the active-site pocket 3.2.1.177 hanging drop method, 2.2 A resolution structure