2.5.1.41 hanging drop vapour diffusion method using 100 mM citrate, pH 6.0, 10 mM MgCl2, 4-10% polyethylene glycol 4000 2.5.1.41 hanging-drop vapor diffusion method. 1.72 A resolution crystal structure of the enzyme in complex with glycerol and sulfate 2.5.1.41 sitting-drop vapor-diffusion method at 20°C, crystal structure of the enzyme in complex with substrate sn-glycerol-1-phosphate is determined at 2.35 A resolution