1.8.2.1	-	
1.8.2.1	crystal structure	
1.8.2.1	the C185S variant is crystallized by the hanging drop vapor diffusion method using 15% (v/v) 2-methyl-2,4-pentanediol, 2% PEG 4000 (w/v), and 100 mM sodium acetate (pH 5.0) and in the presence of sulfite using 2% PEG 4000 (w/v), 10% MPD (v/v), 100 mM sodium acetate (pH 5.0) and 0.5 mM sodium sulfite. The C185A variant is crystallized by the sitting drop vapor diffusion method, using 15% (w/v) PEG 4000, 150 mM (NH4)2SO4, and 100 mM MES (pH 6.0)	
1.8.2.1	vapour diffusion method. Crystals belong to the space group P2(1)2(1)2, with unit-cell parameters a = 97.5, b = 92.5, c = 55.9