1.2.7.1	determination of reduction midpoint potentials from 10 to 85°C. The enzyme exhibits two voltammetric waves with peaks at -280 and -403 mV at room temperature, indicating multiple redox centers, and a single wave at -420 mV at 85°C. Ferredoxin displays a single linear temperature-dependent voltammetric wave at -280 mV at room temperature and -327 mV at 85°C	
1.2.7.1	native structure, those of lactyl-TPP and acetyl-TPP reaction intermediates, and with CoA bound. CoA binds to domain III and CoA binding and domain III movement drive electron transfer from the TPP, which is coordinated by domains I and IV, into the domain V [4Fe-4S] clusters