1.1.1.363	cocrystallization of and mutant enzyme Q365, mutant enzymen S215, mutant enzyme S215 with NAD+ and mutant enzyme Q365 with NADP+, hanging-drop vapour diffusion method, structures of NADP+- and NAD+-complexed enzymes are determined at 2.2 and 2.5 A resolution	
1.1.1.363	crystallized from phosphate buffer in a form suitable for X-ray crystallographic studies. The crystals diffract to better than 2.4 A. The space group is P3(1)21 (P3(2)21), a = 105.8 A, c = 225.1 A, V = 2.18 X 10(6) A(3). The asymmetric unit probably contains a single dimer	
1.1.1.363	hanging drop method, crystallization of six cysteine-containing mutants (Q56C, S61C, S132C, S215C, Q365C, S428C) of the enzyme and the successful preparation and crystallization of a heavy atom derivative of mutant enzyme S215C	
1.1.1.363	hanging drop vapor diffusion technique, determination of the three-dimensional structure of the D177N mutant enzyme by X-ray cryocrystallography in the presence of NAD+ and in the presence of glucose 6-phosphate plus NADPH. The structure of a glucose 6-phosphate complex of a mutant (Q365C) with normal enzyme activity is also determined and substrate binding compared	
1.1.1.363	the three-dimensional structure of the H240N glucose 6-phosphate dehydrogenase is determined at 2.5 A resolution. Crystals are grown in the presence of either glucose 6-phosphate and NAD+ or glucose 6-phosphate and NADP+, hanging drop vapor diffusion method with 2.27 M ammonium sulfate