2.7.1.105 additional information - additional information - 640290 2.7.1.105 additional information - additional information comparison of Ki for MgATP2- inhibition at different free Mg2+ concentrations 640323 2.7.1.105 additional information - additional information comparison of Ki of wild type and mutant enzymes 640330 2.7.1.105 additional information - additional information guanidine-inhibition of wild-type and mutant enzymes 640328 2.7.1.105 additional information - additional information kinetic study 640287 2.7.1.105 additional information - additional information modeling of inhibition of HBP1 and HBP2 by phosphoenolpyruvate and beta-D-fructose 6-phosphate, Km for phosphorylated wild-type enzyme and mutants with beta-D-fructose 6-phosphate and beta-D-fructose 2,6-bisphosphate 660814 2.7.1.105 0.008 - ATP pH 8.2, 30°C, mutant strain DF905 640323 2.7.1.105 0.01 - ATP pH 8.2, 30°C, mutant strain DF903 640323 2.7.1.105 0.014 - phosphoenolpyruvate pH 7.5, 30°C, testis 640330 2.7.1.105 0.016 - glycerate 2-phosphate L form, pH 7.8, 30°C 640315 2.7.1.105 0.018 - phosphoenolpyruvate L form, pH 7.8, 30°C 640315 2.7.1.105 0.018 - sn-glycerol 3-phosphate L form, pH 7.8, 30°C 640315 2.7.1.105 0.02 - glycolate 2-phosphate L form, pH 7.8, 30°C 640315 2.7.1.105 0.025 - 3-(3-pyridinyl)-1-(4-pyridinyl)-2-propen-1-one inhibition of recombinant PFKFB3 protein, no inhibition of purified PFK-1 activity 694113 2.7.1.105 0.025 - citrate hepatoma cells 94932 2.7.1.105 0.026 - citrate liver 640324 2.7.1.105 0.026 - diphosphate L form, pH 7.8, 30°C 640315 2.7.1.105 0.028 - o-phthalaldehyde pH 7.4, room temperature 640309 2.7.1.105 0.029 - glycerate 2-phosphate pH 7.8, 25°C 640345 2.7.1.105 0.035 - citrate - 94932 2.7.1.105 0.045 - phosphoenolpyruvate pH 7.8, 25°C 640345 2.7.1.105 0.08 - phosphoenolpyruvate pH 7.5, 25°C, islet 94948 2.7.1.105 0.08 - phosphoenolpyruvate pH 7.5, 30°C, liver 640330 2.7.1.105 0.083 - citrate pH 7.1, 30°C, native enzyme 94936 2.7.1.105 0.084 - citrate pH 7.1, 30°C, phosphorylated enzyme 94936 2.7.1.105 0.084 - Glycerate 3-phosphate pH 7.8, 25°C 640345 2.7.1.105 0.095 - 2,5-anhydro-D-mannitol 6-phosphate pH 7.5, 30°C 640297 2.7.1.105 0.1 - phosphoenolpyruvate pH 7.5, 30°C, heart 640330 2.7.1.105 0.106 - diphosphate pH 7.8, 25°C 640345 2.7.1.105 0.12 - citrate pH 7.4, 30°C 640341 2.7.1.105 0.12 - phosphoenolpyruvate hepatoma cells 94932 2.7.1.105 0.13 - citrate pH 7.1, 30°C, phosphorylated enzyme 94936 2.7.1.105 0.13 - phosphoenolpyruvate pH 6.0, 25°C 640338 2.7.1.105 0.14 - citrate skeletal muscle 640324 2.7.1.105 0.14 - glycerate 2-phosphate H form, pH 7.8, 30°C 640315 2.7.1.105 0.15 - citrate heart 640324 2.7.1.105 0.15 - phosphoenolpyruvate H form, pH 7.8, 30°C 640315 2.7.1.105 0.15 - sn-glycerol 3-phosphate pH 7.1 640292 2.7.1.105 0.16 - ADP pH 6.6, 25°C 640284 2.7.1.105 0.16 - diphosphate H form, pH 7.8, 30°C 640315 2.7.1.105 0.16 - sn-glycerol 3-phosphate H form, pH 7.8, 30°C 640315 2.7.1.105 0.17 - phosphoenolpyruvate noncompetitive to ATP, pH 7.5, 30°C 640310 2.7.1.105 0.18 - glycolate 2-phosphate H form, pH 7.8, 30°C 640315 2.7.1.105 0.2 - citrate liver 94932 2.7.1.105 0.2 - phosphoenolpyruvate liver 94932 2.7.1.105 0.23 - phosphoenolpyruvate pH 7.4, 30°C 640341 2.7.1.105 0.25 - dihydroxyacetone phosphate L form, pH 7.8, 30°C 640315 2.7.1.105 0.35 - sn-glycerol 3-phosphate pH 6.0, 25°C 640338 2.7.1.105 0.45 - glycerate 2-phosphate pH 6.0, 25°C 640338 2.7.1.105 0.49 - citrate pH 7.1, 30°C, native enzyme 94936 2.7.1.105 0.53 - phosphoenolpyruvate noncompetitive to fructose 6-phosphate, pH 7.5, 30°C 640310 2.7.1.105 0.55 - citrate pH 7.5, 30°C 640310 2.7.1.105 0.7 - phosphoenolpyruvate 30°C 661698 2.7.1.105 0.737 - dihydroxyacetone phosphate pH 7.8, 25°C 640345 2.7.1.105 1.35 - dihydroxyacetone phosphate pH 6.0, 25°C 640338 2.7.1.105 1.9 - dihydroxyacetone phosphate H form, pH 7.8, 30°C 640315 2.7.1.105 2 - N-bromoacetylethanolamine pH 7.5, 30°C, recombinant testis enzyme 640336 2.7.1.105 2 - phosphoenolpyruvate 30°C 94928 2.7.1.105 2.3 - phosphoenolpyruvate pH 6.6, 25°C 640284 2.7.1.105 6 - citrate 30°C 94928 2.7.1.105 8.07 - sn-glycerol 3-phosphate pH 7.8, 25°C 640345 2.7.1.105 8.5 - beta-D-fructose 6-phosphate pH 7.5, 30°C, recombinant HBP1 mutant S460D/T470D 660814 2.7.1.105 9.2 - beta-D-fructose 6-phosphate pH 7.5, 30°C, recombinant HBP1 mutant S460D 660814 2.7.1.105 9.5 - beta-D-fructose 6-phosphate pH 7.5, 30°C, recombinant HBP1 mutant H253A/S460D 660814 2.7.1.105 9.6 - beta-D-fructose 6-phosphate pH 7.5, 30°C, recombinant His-tagged HBP1 deletion mutant 660814 2.7.1.105 9.7 - beta-D-fructose 6-phosphate pH 7.5, 30°C, recombinant HBP1 mutant S477D 660814 2.7.1.105 9.9 - beta-D-fructose 6-phosphate pH 7.5, 30°C, recombinant wild-type HBP1 660814 2.7.1.105 10.9 - beta-D-fructose 6-phosphate pH 7.5, 30°C, recombinant His-tagged HBP1 mutant S302R 660814 2.7.1.105 11.1 - beta-D-fructose 6-phosphate pH 7.5, 30°C, recombinant HBP1 mutant H253A 660814 2.7.1.105 11.8 - beta-D-fructose 6-phosphate pH 7.5, 30°C, recombinant His-tagged wild-type HBP2 660814 2.7.1.105 12.2 - beta-D-fructose 6-phosphate pH 7.5, 30°C, recombinant His-tagged wild-type HBP1 660814 2.7.1.105 12.7 - beta-D-fructose 6-phosphate pH 7.5, 30°C, recombinant non-phosphorylated, His-tagged chimeric mutant enzyme 660814 2.7.1.105 13 - beta-D-fructose 6-phosphate pH 7.5, 30°C, recombinant HBP1 mutant T470 660814 2.7.1.105 13.4 - beta-D-fructose 6-phosphate pH 7.5, 30°C, recombinant phosphorylated, His-tagged chimeric mutant enzyme 660814 2.7.1.105 15 - N-bromoacetylethanolamine pH 7.5, 30°C, crude liver extracts 640336