2.1.1.45	additional information	-	additional information	DHFR inhibition kinetics	742057	
2.1.1.45	0.00000006	-	D1694	-	485413	
2.1.1.45	0.000000095	-	methotrexate	pH 7.4, 25°C, recombinant enzyme	743569	
2.1.1.45	0.000000597	-	trimetrexate	pH 7.4, 25°C, recombinant enzyme	743569	
2.1.1.45	0.000001	-	5-fluoro-2'-deoxyuridylic acid	-	485414	
2.1.1.45	0.000001	-	D1694 pentaglutamate	-	485413	
2.1.1.45	0.0000015	-	D1694 pentaglutamate	-	485413	
2.1.1.45	0.0000017	-	5-fluoro-2'-deoxyuridylic acid	-	485413	
2.1.1.45	0.000002	-	5-fluoro-2'-deoxyuridine 5'-monophosphate	22°C	694812	
2.1.1.45	0.000002	-	5-fluoro-2'-deoxyuridine 5'-monophosphate	inhibited both ThyA and ThyX	694812	
2.1.1.45	0.000002	-	5-fluoro-2'-deoxyuridylic acid	-	485413	
2.1.1.45	0.0000028	-	D1694	-	485413	
2.1.1.45	0.000004	-	5-fluoro-2'-deoxyuridylic acid	-	485358	
2.1.1.45	0.000004	-	Cibacron blue	-	485384	
2.1.1.45	0.000005	-	5-fluoro-2'-deoxyuridylic acid	-	485384, 485393, 485411	
2.1.1.45	0.0000059	-	propylene diphosphonate	wild type enzyme, in 50 mM Tris-HCl, pH 7.4, 1 mM EDTA, 0.2% 2-mercaptoethanol, at 22°C	720995	
2.1.1.45	0.00000635	-	pyrimethamine	pH 7.4, 25°C, recombinant enzyme	743569	
2.1.1.45	0.00001	-	5-Formyl-2'-deoxyuridylic acid	-	485375	
2.1.1.45	0.00001	-	5-Mercapto-2'-deoxyuridylic acid	-	485415	
2.1.1.45	0.00001	-	5-trifluoromethyl-2'-deoxyuridylic acid	-	485375	
2.1.1.45	0.0000113	-	propylene diphosphonate	mutant enzyme A191K, in 50 mM Tris-HCl, pH 7.4, 1 mM EDTA, 0.2% 2-mercaptoethanol, at 22°C	720995	
2.1.1.45	0.000012	-	5-Formyl-2'-deoxyuridylic acid	-	485365	
2.1.1.45	0.000013	-	5-Formyl-2'-deoxyuridylic acid	-	485365	
2.1.1.45	0.000014	-	5-fluoro-2'-deoxyuridine 5'-monophosphate	-	485378	
2.1.1.45	0.0000176	-	trimethoprim	pH 7.4, 25°C, recombinant enzyme	743569	
2.1.1.45	0.000018	-	(2R)-2-(5-[[(1-hydroxy-3-methyl-5,10a-dihydrobenzo[f]quinazolin-9-yl)methyl]amino]-1-oxo-1,3-dihydro-2H-isoindol-2-yl)butanedioic acid	22°C	694812	
2.1.1.45	0.000018	-	GW1843U89	-	694812	
2.1.1.45	0.00002	-	5-fluoro-2'-deoxyuridylic acid	L1210 parental enzyme	485427	
2.1.1.45	0.000021	-	5-fluoro-2'-deoxyuridylic acid	50 nM	485404	
2.1.1.45	0.0000235	-	5-fluoro-2'-deoxyuridylic acid	-	485404	
2.1.1.45	0.000026	-	5-fluoro-2'-deoxyuridylic acid	200 nM	485404	
2.1.1.45	0.000029	-	5-Nitro-2'-deoxyuridine 5'-monophosphate	-	485378	
2.1.1.45	0.000038	-	ZD9331	pH 7.2, 22°C	690892	
2.1.1.45	0.000039	-	5-trifluoromethyl-2'-deoxyuridine 5'-monophosphate	-	485378	
2.1.1.45	0.00004	-	5-Mercapto-2'-deoxyuridylic acid	-	485375	
2.1.1.45	0.00005	-	5-fluoro-2'-deoxyuridylic acid	-	485389	
2.1.1.45	0.00009	-	CB3717	-	485420	
2.1.1.45	0.0000931	-	raltitrexed	pH 7.4, 25°C, recombinant enzyme	743569	
2.1.1.45	0.0001	-	10-Formyltetrahydrofolylheptaglutamate	-	485398	
2.1.1.45	0.0001	-	5-fluoro-2'-deoxyuridine 5'-monophosphate	22°C	694812	
2.1.1.45	0.0001	-	Procion blue	variable substrate 2'-deoxyuridine	485393	
2.1.1.45	0.00012	-	tetrahydromethotrexate	-	485373	
2.1.1.45	0.00022	-	Procion blue	variable substrate 5,10-methylenetetrahydropteroylglutamate	485393	
2.1.1.45	0.00029	-	pemetrexed	pH 7.4, 25°C, recombinant enzyme	743569	
2.1.1.45	0.0003	-	chloro-2'-deoxyuridine	L1210 parental enzyme	485427	
2.1.1.45	0.000348	-	nolatrexed	pH 7.4, 25°C, recombinant enzyme	743569	
2.1.1.45	0.0006	-	pteroyltetraglutamate	-	485395	
2.1.1.45	0.0007	-	10-formyldihydropteroylheptaglutamate	-	485398	
2.1.1.45	0.0008	-	1-(beta-D-2-deoxyribofuranosyl)-4-hydroxyamino-5-fluoropyrimidin-2(1H)-one 5'-monophosphate	L1210 parental enzyme	485427	
2.1.1.45	0.000833	-	trimethoprim	1.0 mM	485404	
2.1.1.45	0.00087	-	trimethoprim	2.0 mM	485404	
2.1.1.45	0.001	-	pteroylpentaglutamate	-	485395	
2.1.1.45	0.001	-	thymidine phosphate	0.25 mM, noncompetitive inhibition, 5,10-mehylenetetrahydrofolate as variable substrate	485404	
2.1.1.45	0.0011	-	5-fluoro-2'-deoxyuridine-5'-monophosphate	wild type enzyme, in 50 mM TES buffer, 50 mM, MgCl2, 13 mM formalin, 2 mM EDTA, 0.15 mM 2-mercaptoethanol, pH 7.4, at 25°C	720965	
2.1.1.45	0.00124	-	thymidine phosphate	1.0 mM, noncompetitive inhibition, 5,10-methylenetetrahydrofolate as variable substrate	485404	
2.1.1.45	0.0013	-	1-(beta-D-2-deoxyribofuranosyl)-4-hydroxyamino-5-hydroxymethylpyrimidin-2(1H)-one 5'-monophosphate	L1210 recombinant enzyme	485427	
2.1.1.45	0.0013	-	2,4-diamino quinazoline	-	485372	
2.1.1.45	0.0013	-	SK 28,757	-	485372	
2.1.1.45	0.0014	-	1-(beta-D-2-deoxyribofuranosyl)-4-hydroxyamino-5-fluoropyrimidin-2(1H)-one 5'-monophosphate	L1210 recombinant enzyme	485427	
2.1.1.45	0.0014	-	dihydrofolate	1.0 mM, noncompetitive inhibition, 5,10-methylenetetrahydrofolate as variable substrate	485404	
2.1.1.45	0.0015	-	bromo-2'-deoxyuridine	L1210 parental enzyme	485427	
2.1.1.45	0.0015	-	SK 28,758	-	485372	
2.1.1.45	0.0016	-	10-formyldihydropteroylpentaglutamate	-	485398	
2.1.1.45	0.0017	-	1-(beta-D-2-deoxyribofuranosyl)-4-hydroxyaminopyrimidin-2(1H)-one 5'-monophosphate	L1210 parental enzyme	485427	
2.1.1.45	0.0017	-	thiophosphate	-	485430	
2.1.1.45	0.00178	-	dihydrofolate	5.0 mM, noncompetitive inhibition, 5,10-methylenetetrahydrofolate as variable substrate	485404	
2.1.1.45	0.00191	-	1-(beta-D-2-deoxyribofuranosyl)-4-hydroxyamino-5-hydroxymethylpyrimidin-2(1H)-one 5'-monophosphate	L1210 parental enzyme	485427	
2.1.1.45	0.002	-	2-(4-hydroxy[1,1'-biphenyl]-3-yl)-4-methyl-1H-isoindole-1,3(2H)-dione	pH not specified in the publication, temperature not specified in the publication	756644	
2.1.1.45	0.002	-	5-fluoro-2'-deoxyuridylic acid	-	485413	
2.1.1.45	0.002	-	pteroylhexaglutamate	-	485395	
2.1.1.45	0.0024	-	10-Formyltetrahydrofolylmonoglutamate	-	485398	
2.1.1.45	0.0027	-	1,3-propanediphosphonic acid	pH 7.2, 22°C	690892	
2.1.1.45	0.0029	-	methasquin	-	485372	
2.1.1.45	0.0029	-	SK 28,815	-	485372	
2.1.1.45	0.00294	-	dihydrofolate	0.5 mM, noncompetitive inhibition, dUMP as variable substrate	485404	
2.1.1.45	0.003	-	2,4-diamino quinazoline antifolate	-	485372	
2.1.1.45	0.00303	-	dihydrofolate	1.0 mM, noncompetitive inhibition, dUMP as variable substrate	485404	
2.1.1.45	0.0034	-	1-(beta-D-2-deoxyribofuranosyl)-4-hydroxyamino-5-iodopyrimidin-2(1H)-one 5'-monophosphate	L1210 recombinant enzyme	485427	
2.1.1.45	0.0034	-	Pteroylheptaglutamate	-	485395	
2.1.1.45	0.0034	-	Pteroyltriglutamate	-	485395	
2.1.1.45	0.0037	-	hydroxymethyl-2'-deoxyuridine	L1210 parental enzyme	485427	
2.1.1.45	0.0044	-	quinaspar	-	485372	
2.1.1.45	0.00477	-	2',5-difluoro-ara-UMP	-	702544	
2.1.1.45	0.0049	-	1-(beta-D-2-deoxyribofuranosyl)-4-hydroxyamino-5-chloropyrimidin-2(1H)-one 5'-monophosphate	L1210 recombinant enzyme	485427	
2.1.1.45	0.0054	-	5-bromoacetamido-5'-deoxythymidine	competitive inhibition	485391	
2.1.1.45	0.0054	-	5-fluoro-2'-deoxyuridine-5'-monophosphate	mutant enzyme E30W, in 50 mM TES buffer, 50 mM, MgCl2, 13 mM formalin, 2 mM EDTA, 0.15 mM 2-mercaptoethanol, pH 7.4, at 25°C	720965	
2.1.1.45	0.0066	-	5-bromoacetamido-5'-deoxythymidine	-	485391	
2.1.1.45	0.0073	-	thymidine phosphate	L1210 parental enzyme	485427	
2.1.1.45	0.00746	-	1-(beta-D-2-deoxyribofuranosyl)-4-hydroxyamino-5-iodopyrimidin-2(1H)-one 5'-monophosphate	L1210 parental enzyme	485427	
2.1.1.45	0.0075	-	10-formyldihydropteroylglutamate	-	485398	
2.1.1.45	0.0075	-	deaza-aminopterin	-	485372	
2.1.1.45	0.0076	-	pteroylmonoglutamate	-	485395	
2.1.1.45	0.00768	-	iodo-2'-deoxyuridine	L1210 parental enzyme	485427	
2.1.1.45	0.0084	-	dithiophosphate	-	485430	
2.1.1.45	0.0085	-	1-(beta-D-2-deoxyribofuranosyl)-4-hydroxyamino-5-chloropyrimidin-2(1H)-one 5'-monophosphate	L1210 parental enzyme	485427	
2.1.1.45	0.0093	-	1-(beta-D-2-deoxyribofuranosyl)-4-hydroxyaminopyrimidin-2(1H)-one 5'-monophosphate	L1210 recombinant enzyme	485427	
2.1.1.45	0.01	-	5-bromo-2'-deoxyuridine 5'-monophosphate	-	485384	
2.1.1.45	0.0103	-	2'-fluoro-ara-UMP	-	702544	
2.1.1.45	0.013	-	2-[2-(3-methoxyphenoxy)propyl]-4-methyl-1H-isoindole-1,3(2H)-dione	pH not specified in the publication, temperature not specified in the publication	756644	
2.1.1.45	0.0132	-	pteroyldiglutamate	-	485395	
2.1.1.45	0.015	-	aminopterin	-	485384	
2.1.1.45	0.016	-	1-(2'-deoxyribosyl)-8-azapurin-2-one 5'-monophosphate	-	485385	
2.1.1.45	0.02	-	10-formyltetrahydropteroylmonoglutamate	-	485398	
2.1.1.45	0.02	-	aminopterin	-	485384	
2.1.1.45	0.02	-	methotrexate	-	485384	
2.1.1.45	0.022	-	1-(beta-D-2-deoxyribofuranosyl)-4-hydroxyamino-5-bromopyrimidin-2(1H)-one 5'-monophosphate	L1210 recombinant enzyme	485427	
2.1.1.45	0.023	-	methopterin	-	485393	
2.1.1.45	0.023	-	methotrexate	uncompetitive with methylene tetrahydrofolate	485358	
2.1.1.45	0.024	-	4,5,6,7-tetrachloro-2-[3-(phenylsulfanyl)propyl]-1H-isoindole-1,3(2H)-dione	pH not specified in the publication, temperature not specified in the publication	756644	
2.1.1.45	0.024	-	dTMP	-	485358, 485363	
2.1.1.45	0.025	-	4'-[(4-methyl-1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)methyl][1,1'-biphenyl]-2-carbonitrile	pH not specified in the publication, temperature not specified in the publication	756644	
2.1.1.45	0.0284	-	methopterin	-	485372	
2.1.1.45	0.03	-	2,4-diamino pteridine	-	485372	
2.1.1.45	0.0321	-	1-(beta-D-2-deoxyribofuranosyl)-4-hydroxyamino-5-bromopyrimidin-2(1H)-one 5'-monophosphate	L1210 parental enzyme	485427	
2.1.1.45	0.0347	-	aminopterin	-	485372	
2.1.1.45	0.059	-	dTMP	-	485363	
2.1.1.45	0.07	-	dTMP	variable substrate 2'-deoxyuridine	485393	
2.1.1.45	0.073	-	methopterin	variable substrate 2'-deoxyuridine	485393	
2.1.1.45	0.08	-	thymidine phosphate	-	485384	
2.1.1.45	0.1	-	dTMP	-	485384	
2.1.1.45	0.103	-	methotrexate	uncompetitive with dUMP	485389	
2.1.1.45	0.12	-	1-(beta-D-2'-deoxyribofuranosyl)-1,6-dihydro-8-azapurin-2(3H,6H)-one 5'-monophosphate	-	485385	
2.1.1.45	0.12	-	thymidine phosphate	-	485384	
2.1.1.45	0.14	-	dTMP	-	485363	
2.1.1.45	0.238	-	methotrexate	0.05 mM	485404	
2.1.1.45	0.24	-	dTMP	variable substrate 5,10-methylenetetrahydropteroylglutamate	485393	
2.1.1.45	0.267	-	methotrexate	0.02 mM	485404	
2.1.1.45	0.3125	-	thymidine phosphate	0.25 mM, competitive inhibition, dUMP as variable substrate	485404	
2.1.1.45	0.333	-	thymidine phosphate	-	485404	
2.1.1.45	0.406	-	methotrexate	0.5 mM	485404	
2.1.1.45	0.413	-	methotrexate	1.0 mM	485404	
2.1.1.45	0.4328	-	2'-deoxy-2',2'-difluorouridine 5'-dihydrogen	-	702544	
2.1.1.45	0.6	0.9	pyridoxal 5'-phosphate	-	485396	
2.1.1.45	0.852	-	trimethoprim	-	485404	
2.1.1.45	5	-	aminofolate	1.0 mM	485404	
2.1.1.45	5.2	-	aminofolate	5.0 mM	485404	
2.1.1.45	12	-	glyceraldehyde 3-phosphate	-	485399