5.3.99.3 glutathione - 652244, 653753, 662071, 693372, 693551, 693841, 694863, 705347, 714468, 714944, 715541, 715865, 716081 5.3.99.3 glutathione dependent on 749180 5.3.99.3 heme crystallization data of red-coloured protein purified after growth of recombinant Escherichia coli in LB medium containing delta-aminolevulinate and Fe(NO3)3. Heme is complexed with bound glutathione forming a S-Fe coordination bond with no polar interaction with enzyme. Heme dissociation constant is 0.00053 mM 678310 5.3.99.3 additional information mutants R126A/Q possess glutathione-dependent reductase activity 693240