1.2.4.1 NAD+ - 654462, 654647, 654734, 654741, 655474, 657068, 663808, 684365, 685160, 685585, 686469, 686772, 697756, 698954, 700236, 710839, 712851 1.2.4.1 NAD+ required 726840 1.2.4.1 thiamine diphosphate - 348949, 348951, 348968, 348974, 348981, 654462, 654734, 657000, 657068, 671044, 684365, 685585, 686469, 686772, 687226, 690077, 710839, 712851, 741339, 741340 1.2.4.1 thiamine diphosphate dependent 675426, 685160, 687613, 689791 1.2.4.1 thiamine diphosphate dependent on 725205, 725521, 726840 1.2.4.1 thiamine diphosphate dependent on, 2 bound in the V-conformation in clefts between the 2 subunits, completely buried in the structure, binding structure 654647 1.2.4.1 thiamine diphosphate dependent on, binding site 655474 1.2.4.1 thiamine diphosphate dependent on, binding structure and determinants, V conformation, K392 is important 654741 1.2.4.1 thiamine diphosphate kcat/Km value for wild-type 20 per mM and s, for mutant H271A 950 per mM and s 735664 1.2.4.1 thiamine diphosphate KD value 0.00047 mM 672086