1.14.12.22 FAD 1 mol of His-tagged CarAd contains 1 mol of FAD 707219 1.14.12.22 Ferredoxin ferredoxin component CarAc acts as a mediator in the electron transfer from ferredoxin reductase CarAd to terminal oxygenase CarAa. It contains a Rieske [2Fe-2S] cluster, which is located at the tip of the cluster-binding domain, where it is exposed to solvent 710504 1.14.12.22 Ferredoxin ferredoxin component contains two Rieske [2Fe-2S] clusters 710576 1.14.12.22 NAD(P)H - 707220, 707776, 707814, 707821, 708908, 710385 1.14.12.22 NADH - 741525, 741682, 742172, 742964 1.14.12.22 NADH both NADH and NADPH are effective as electron donors for His-tagged ferrdoxin reductase CarAd. The ratio kcat/Km for NADH is 22.3-fold higher than that for NADPH in the 2,6-dichlorophenolindophenol reductase assay 707219 1.14.12.22 NADPH - 741525, 741682, 742172, 742964 1.14.12.22 NADPH both NADH and NADPH are effective as electron donors for His-tagged ferrdoxin reductase CarAd. The ratio kcat/Km for NADH is 22.3-fold higher than that for NADPH in the 2,6-dichlorophenolindophenol reductase assay 707219 1.14.12.22 [2Fe-2S]-center - 741682