5.3.99.3 glutathione - 5.3.99.3 glutathione dependent on 5.3.99.3 heme crystallization data of red-coloured protein purified after growth of recombinant Escherichia coli in LB medium containing delta-aminolevulinate and Fe(NO3)3. Heme is complexed with bound glutathione forming a S-Fe coordination bond with no polar interaction with enzyme. Heme dissociation constant is 0.00053 mM 5.3.99.3 additional information mutants R126A/Q possess glutathione-dependent reductase activity