1.4.1.13 FAD - 676332, 725401 1.4.1.13 FAD the isoalloxazine ring is almost solvent inaccessible, neighboring residues are Ile98, Pro100, Leu186, Ile191, Lys195, Leu266, Asp300, Thr301, Asp304, Leu450 and Val451, together with backbone atoms of the surrounding regions 763361 1.4.1.13 flavin 0.83 mol FAD and 0.86 mol FMN per mol alphabeta protomer 391467 1.4.1.13 flavin 0.83 mol of FMN and 0.81 mol of FAD per mol of enzyme 391459 1.4.1.13 flavin 0.86 mol FAD per mol of mutant beta subunit, 0.83 mol FAD per mol of the wild type species 391466 1.4.1.13 flavin 2 flavin moieties per 220000 MW 391449 1.4.1.13 flavin contains FAD and FMN 391438, 391440, 391444, 391446, 391467, 391468, 391469 1.4.1.13 flavin enzyme contains 2 mol of FMN and 2 mol of FAD 391447 1.4.1.13 flavin enzyme contains 7.8 mol of flavin 391438 1.4.1.13 flavin flavoenzyme 391438, 391444, 391446, 391452, 391455, 391462, 391463, 391467, 391468, 391469 1.4.1.13 flavin ratio FAD: FMN is 1 391446, 391468 1.4.1.13 flavin ratio FAD: FMN is 1.1 391440 1.4.1.13 flavin ratio FAD: FMN is 1.2 391438, 391449 1.4.1.13 flavin the alpha subunit contains FMN as flavin cofactor, 0.94 FMN bound per alpha subunit 391465 1.4.1.13 flavin the beta subunit contains the binding site for FAD, 0.83 mol FAD per mol beta subunit 391463, 391465 1.4.1.13 FMN - 676332 1.4.1.13 additional information midpoint potential value of the FMN cofactor: approximately -240 mV, midpoint potential value of the 3Fe-4S cluster: approximately -270 mV, midpoint potential value of the FAD cofactor: approximately -340 mV for the beta subunit and -300 mV for the holoenzyme 391468 1.4.1.13 additional information purified enzyme contains 30.4 mol of labile sulfide per 800,000 g of protein 391438 1.4.1.13 additional information the alpha subunit contains the [3Fe-4S] cluster of the enzyme 391465 1.4.1.13 additional information the enzyme contains 7.2 mol of acid-labile sulfur per 200,000 g of protein 391440 1.4.1.13 additional information the enzyme contains 7.9 sulfur atoms per protomer with a molecular weight of 185000 391446 1.4.1.13 additional information the enzyme contains 8.1 acid-labile sulfur atoms per 220000-dalton dimer 391449 1.4.1.13 additional information the enzyme contains 8.7 g atoms per mol 391459 1.4.1.13 additional information the enzyme contains three different iron-sulfur clusters, one 3Fe-4S center on the alpha subunit and two 4Fe-4S clusters of unknown location, 11.7 mol sulfur per mol alphabeta protomer 391467 1.4.1.13 additional information the enzyme contains three distinct ion-sulfur centers per alphabeta protomer 391463 1.4.1.13 additional information the FMN and FAD prosthetic groups are demonstrated to be nonequivalent with respect to their reactivities with sulfite, sulfite reacts with only one of the two flavins forming an N(5)-sulfite adduct 391444 1.4.1.13 additional information thio-NADPH and acetylpyridine-NADPH can be used as electron donors but are less efficient than NADPH 391445 1.4.1.13 NADH 21% relative activity to NADPH 391459 1.4.1.13 NADH no activity with NADH 391438, 391440, 391443, 391446, 391447, 391451, 391452, 391455, 391460, 391461, 391462 1.4.1.13 NADP+ - 725886 1.4.1.13 NADPH - 391438, 391439, 391440, 391441, 391442, 391443, 391444, 391446, 391447, 391448, 391449, 391450, 391451, 391452, 391453, 391454, 391455, 391456, 391457, 391458, 391459, 391460, 391461, 391462, 391464, 391467, 391468, 391469, 674303, 725401, 725886 1.4.1.13 NADPH oxidizes NADPH stereospecifically at the 4S position 391445 1.4.1.13 NADPH the beta subunit contains the NADPH binding site of the enzyme 391463, 391465 1.4.1.13 NADPH the C-terminal potential ADP-binding fold of the beta subunit is the NADPH-binding site of the enzyme 391466 1.4.1.13 [4Fe-4S]-center the [4Fe-4S]+1,+2 cluster A displays three Cys and one Glu ligands for the Fe atoms. Residues Cys105 and Cys60 are linked to Fe1 and Fe2, respectively and the Fe3 atom shows bidentate coordination to Glu125 carboxylate, Fe4 is coordinated to Cys99, whose Calpha atom falls 5 A from FAD dimethyl-benzene ring C8 methyl. All Cys ligands to the [4Fe-4S]+1,+2 cluster B (Cys48, Cys51, Cys56, Cys109) are comprised in small subunit GltD loops at the interface with GltB 763361