1.4.1.13 flavin ratio FAD: FMN is 1 391446, 391468 1.4.1.13 flavin ratio FAD: FMN is 1.1 391440 1.4.1.13 flavin ratio FAD: FMN is 1.2 391438, 391449 1.4.1.13 flavin the alpha subunit contains FMN as flavin cofactor, 0.94 FMN bound per alpha subunit 391465 1.4.1.13 flavin the beta subunit contains the binding site for FAD, 0.83 mol FAD per mol beta subunit 391463, 391465 1.4.1.13 FMN - 676332 1.4.1.13 additional information midpoint potential value of the FMN cofactor: approximately -240 mV, midpoint potential value of the 3Fe-4S cluster: approximately -270 mV, midpoint potential value of the FAD cofactor: approximately -340 mV for the beta subunit and -300 mV for the holoenzyme 391468 1.4.1.13 additional information purified enzyme contains 30.4 mol of labile sulfide per 800,000 g of protein 391438 1.4.1.13 additional information the alpha subunit contains the [3Fe-4S] cluster of the enzyme 391465 1.4.1.13 additional information the enzyme contains 7.2 mol of acid-labile sulfur per 200,000 g of protein 391440