1.17.1.9 NADP+ - 725222 1.17.1.9 NADP+ 4.2% of the activity with NAD+ 655047 1.17.1.9 NADP+ activity is 2.4% of the activity with NAD+ 655057 1.17.1.9 NADP+ FDH1 shows activity using NAD+ or NADP+, FDH1 is highly specific to NAD+ compared to NADP+ 696475 1.17.1.9 NADP+ low activity compared to NADP+ 686705 1.17.1.9 NADP+ no reaction is catalyzed with wild-type enzyme, activity is detected with mutant enzyme D195S. The ratio of the catalytic efficiencies for NAD+ versus NADP+ for the mutant protein is 40:1 in favor of NAD+ 288122 1.17.1.9 NADP+ the enzyme prefers to use NAD+ as an electron acceptor more than NADP+ 763261 1.17.1.9 NADP+ the wild type enzyme virtually fails to use NADP+ as a cofacor 686705 1.17.1.9 NADP+ wild-type enzyme shows no activity with NADP+, mutant enzyme D196A/Y197R shows higher activity with NADP+ than with NAD+ 654505 1.17.1.9 [2Fe-2S]-center - 740431