1.1.1.47 additional information - sharp drop in activity at alkaline pH of wild-type and mutant Q252L activities, not mutant Q252L/E170K 654301 1.1.1.47 4 10.5 - 711501 1.1.1.47 4.5 11 50% of maximal activity at pH 4.5 and pH 11.0 656567 1.1.1.47 5 8 when incubated for 6 h at 37°C, the enzyme is highly stable in acidic conditions (pH 5.0-6.0) but is completely inactivated at pH values above 8.0 739842 1.1.1.47 5.5 10.5 the enzyme's activity is more than 60% of its maximum at pH values ranging from 5.5 to 10.5 740382 1.1.1.47 6 9.5 pH 6.0: about 40% of maximal activity, pH 9.5: about 45% of maximal activity 639121 1.1.1.47 7 8.5 pH 7.0: 70% of maximal activity with glucose and NAD+ and about 55% of the activity with glucose and NADP+, pH 8.5: about 55% of maximal activity with glucose and NAD+ and about 75% of the activity with glucose and NADP+ 639124 1.1.1.47 7 10 more than 60% activity between pH 7.0 and 10.0 740857 1.1.1.47 7 10 pH 7.0: about 40% of maximal activity, pH 10.0: about 45% of maximal activity 763151 1.1.1.47 7.5 10.5 at pH 7.5 and 10.0 respectively 75% and 50% activity compared to optimal pH 639119