6.5.1.3 5 9.5 activity is virtually nil at pH below 5.0 or above 9.5 654015 6.5.1.3 5.5 8 adenylyltransferase activity 653679 6.5.1.3 5.5 8.5 nick sealing activity is optimal at pH 5.5 to 6.5 and declined sharply at pH 8.5 or above 749277 6.5.1.3 5.7 7 - 650172 6.5.1.3 6.5 8 around 70% of the maximum at pH 8.0 651051 6.5.1.3 7 8.5 pH 7.0: about 40% of maximal activity, pH 8.5: about 30% of maximal activity 662976