3.5.4.2 4 8 - 3.5.4.2 5 8.5 pH 5: about 30% of maximal activity, pH 8.5: about 20% of maximal activity 3.5.4.2 6 8 potassium phosphate buffer, maximal activity pH 7 3.5.4.2 7 9 Tris-HCl buffer, maximal activity pH 9 3.5.4.2 7.5 9 glycine-NaOH buffer, maximal activity pH 7