3.4.22.51 additional information - substrates placing hydrophobic residues in the specificity pocket are cleaved at a broader pH range than hydrophilic substrates 638882 3.4.22.51 4.5 - hydrolysis of 2-aminobenzoyl-FRA-(2,4-dinitrophenyl)-epsilon-NH2-lysine-OH 668458 3.4.22.51 5 - assay with azocasein 638878 3.4.22.51 5.5 - room temperature, 45 min 699388 3.4.22.51 6 - - 638883, 638884 3.4.22.51 6 - assay with beta-naphthylamide 638875 3.4.22.51 6 7 hydrolysis of 2-aminobenzoyl-FRA-(2,4-dinitrophenyl)-epsilon-NH2-lysine-NH2 668458 3.4.22.51 6.5 - assay at 679892, 717894, 717917, 753924 3.4.22.51 6.5 - assay at 28°C 697939 3.4.22.51 6.5 - assay at room temperature, 5 min, 0.64 nM cruzain, 50 mM sodium phosphate, 100 mM sodium chloride, 5 mM EDTA 696710