6.3.5.5 4.2 - and a second optimum at pH 9.5, glutaminase activity 1725 6.3.5.5 4.2 - wild-type enzyme 649994 6.3.5.5 6.6 - mutant enzyme E841K 649994 6.3.5.5 6.7 - highest activity in the pH-range 6.7-8.8 1726 6.3.5.5 6.8 7.2 Gln-dependent activity 1735 6.3.5.5 7.5 - with either 5 mM Gln or 100 mM NH4+ as substrate 1743 6.3.5.5 7.6 - assay at 693247, 695002, 704173 6.3.5.5 7.6 8.5 Gln-dependent activity 1736 6.3.5.5 7.8 - NH4+-dependent activity 1735 6.3.5.5 7.8 8.2 - 1725, 1731 6.3.5.5 8 - assay at 690647 6.3.5.5 8 - triethanolamine buffer 1733 6.3.5.5 9.3 - wild-type enzyme 649994 6.3.5.5 9.5 - and a second optimum at pH 4.2, glutaminase activity 1725