3.4.22.51	additional information	-	substrates placing hydrophobic residues in the specificity pocket are cleaved at a broader pH range than hydrophilic substrates	638882	
3.4.22.51	4.5	-	hydrolysis of 2-aminobenzoyl-FRA-(2,4-dinitrophenyl)-epsilon-NH2-lysine-OH	668458	
3.4.22.51	5	-	assay with azocasein	638878	
3.4.22.51	5.5	-	room temperature, 45 min	699388	
3.4.22.51	6	-	-	638883, 638884	
3.4.22.51	6	-	assay with beta-naphthylamide	638875	
3.4.22.51	6	7	hydrolysis of 2-aminobenzoyl-FRA-(2,4-dinitrophenyl)-epsilon-NH2-lysine-NH2	668458	
3.4.22.51	6.5	-	assay at	679892, 717894, 717917, 753924	
3.4.22.51	6.5	-	assay at 28°C	697939	
3.4.22.51	6.5	-	assay at room temperature, 5 min, 0.64 nM cruzain, 50 mM sodium phosphate, 100 mM sodium chloride, 5 mM EDTA	696710	
3.4.22.51	6.5	-	complete inhibition by the propeptide of cruzipain	679761	
3.4.22.51	6.8	-	KEEASSAVVGGPG as substrate	638877, 638879	
3.4.22.51	6.8	-	KEEASSAVVRGPG as substrate	638877, 638879	
3.4.22.51	7	-	beta-naphthylamide derivatives as substrate	638878	
3.4.22.51	7.6	-	assay at	717475	
3.4.22.51	7.6	-	assay with 4-nitroanilide	638875	
3.4.22.51	8	-	30 min, 50 mM Tris-acetate buffer, 0.3 mM Bz-Pro-Phe-Arg-pNA, 10 mM beta-mercaptoethanol	696729	
3.4.22.51	8	-	assay at pH 8.0, 60 min, room temperature	701365	
3.4.22.51	9	-	4-nitroanilide derivatives as substrate	638878