3.4.21.9 additional information - shows pH optima at 8.0-8.5 for Val-(Asp)4-Lys-Ile-Val-Gly and 8.5-9 for Val-(Asp)3-Ala-Lys-Ile-Val-Gly 696841 3.4.21.9 5 - assay at, substrate trypsin 718100 3.4.21.9 5.2 5.7 bovine trypsinogen 95581 3.4.21.9 6 - - 669550 3.4.21.9 6 - immobilised enzyme on paramagnetic microspheres 683266 3.4.21.9 6 9 activity rises sharply between pH 5 and 6 and then remains maximal until pH 9 95575 3.4.21.9 7.5 - - 683172 3.4.21.9 8 - - 716743 3.4.21.9 8 - assay at 717409, 718352 3.4.21.9 8 - assay at, substrate human interferon-alpha2a 718100 3.4.21.9 8 - free enzyme 683266 3.4.21.9 8 - hydrolysis of Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide 95584 3.4.21.9 8 - in presence of 7.5 mM CaCl2 95568 3.4.21.9 8 - with substrate GD4K 7-amido-4-methylcoumarin 717431 3.4.21.9 8.4 - assay at 718359 3.4.21.9 9 - immobilised enzyme on hexamethylamino Sepabeads 683266