2.4.1.19 additional information - the enzymes are more active in glycine/NaOH buffer compared with Na2HPO4/NaH2PO4 buffer at the same pH 719222 2.4.1.19 4 - INMIA 3849 488855 2.4.1.19 4 - starch degrading activity, wild-type enzyme 660462 2.4.1.19 4.5 - - 756523 2.4.1.19 4.5 4.7 - 488839 2.4.1.19 4.5 4.7 var. alkalophilus ATCC 21783, second pH optimum at 7.5-8.5 488855 2.4.1.19 4.5 5 ATCC 21783, crude enzyme, second pH optimum at 8.0-9.0 488826 2.4.1.19 4.5 7 - 488855 2.4.1.19 4.6 - ATCC 21783 contains 3 isozymes, neutral, alkalic and acidic, possessing markedly different pH optima, 4.6, 7.0 and 9.5 488831, 488834 2.4.1.19 5 - - 488855, 755859, 756523 2.4.1.19 5 - ATCC 39612/Ha3-3-2, starch dextrinizing activity, second pH optimum at 9.0 488832 2.4.1.19 5 - free and glutaraldehyde-activated chitosan spheres-immobilized enzyme 735891 2.4.1.19 5 - optimum pH for L-ascorbic acid-2-O-alpha-D-glucoside formation 488841 2.4.1.19 5 - purified enzyme 686621 2.4.1.19 5 5.5 - 488858 2.4.1.19 5 5.7 - 488825, 488832, 488839, 488855 2.4.1.19 5 5.7 IFO 3490 488855 2.4.1.19 5 5.7 No. 5 strain 488825 2.4.1.19 5 6 - 488849 2.4.1.19 5 6 strain BIO-9m 488861 2.4.1.19 5 6.5 strain B-4025 488861 2.4.1.19 5 8 - 488864 2.4.1.19 5.2 - assay at 702790 2.4.1.19 5.2 - strain M5 a1 488827 2.4.1.19 5.4 5.8 - 488829 2.4.1.19 5.5 - - 488835, 488839, 488842, 488851, 701849, 719222, 756523, 757040, 758476 2.4.1.19 5.5 - amylolytic activity assay at 703068 2.4.1.19 5.5 - and a second optimum at pH 9.0 671395 2.4.1.19 5.5 - assay at 668001, 701849 2.4.1.19 5.5 - C31 488835 2.4.1.19 5.5 - enzyme immobilized on glyoxyl-agarose and free enzyme present similar pH/activities profiles, with two peaks at pH values of 5.5 and 7 and a minimum at pH 6.0-6.5 in both amylolytic and CGTase activities 672766 2.4.1.19 5.5 - immobilized enzyme 488830 2.4.1.19 5.5 - strain 1011, wild-type 488851 2.4.1.19 5.5 5.8 strain BIO-3m 488861 2.4.1.19 5.5 5.8 strain E 192 488842 2.4.1.19 5.5 6 - 488863 2.4.1.19 5.5 7 strain B-4018 488861 2.4.1.19 5.5 8.5 - 488856 2.4.1.19 5.7 - assay at 702736 2.4.1.19 5.7 - recombinant enzyme, cgtM gene product 488845 2.4.1.19 5.8 6 - 659612 2.4.1.19 5.9 - - 488830 2.4.1.19 5.9 6.5 the optimum pH for dextrinizing and cyclization activities of purified CGTases from both sources are similar around pH 6.5 and pH 5.9 respectively 687470 2.4.1.19 6 - - 488839, 488845, 488853, 488855, 660343, 673391, 689822, 701849, 736202, 736256, 737189, 755664, 755883, 756523 2.4.1.19 6 - and 8.0 718610 2.4.1.19 6 - and 9.0 720955 2.4.1.19 6 - assay at 685009, 687232, 687691, 701845, 701863, 702844, 702849, 703063, 704134, 704138, 705236 2.4.1.19 6 - cgt-1 gene product 488845 2.4.1.19 6 - cyclization activity 702110 2.4.1.19 6 - immobilized enzyme 684526 2.4.1.19 6 - INMIA A7/1 488855 2.4.1.19 6 - native enzyme 673578 2.4.1.19 6 - strain BC251 enzyme 701849 2.4.1.19 6 - two maxima at pH 6.0 and pH 8.6 684450 2.4.1.19 6 6.5 strain BIO-13m 488861 2.4.1.19 6 7 - 488861 2.4.1.19 6 7 for the product of genistein monoglucoside, the enzyme's yield reaches the highest level at pH 6.5, while the most genistein diglucoside is obtained at pH 7.0 757354 2.4.1.19 6 7 strain BIO-12H 488861 2.4.1.19 6 8 native enzyme 673578 2.4.1.19 6 9 - 657693 2.4.1.19 6.1 6.2 - 488824 2.4.1.19 6.1 6.2 ATCC 8514 488855 2.4.1.19 6.4 - assay at 703496 2.4.1.19 6.5 - - 488855, 684517, 684942, 735493, 736248 2.4.1.19 6.5 - INMIA T6, INMIA T 42 488855 2.4.1.19 6.5 - strain BIO-2m 488861 2.4.1.19 6.5 - wild-type enzyme 659737 2.4.1.19 6.5 7 for the product of genistein monoglucoside, its yield reaches the highest level at pH 6.5, while the most genistein diglucoside is obtained at pH 7.0 757354 2.4.1.19 6.5 7 strain BIO-13H 488861 2.4.1.19 6.5 8 ATCC 39612/Ha3-3-2, cyclodextrin forming activity 488832 2.4.1.19 6.5 8.5 - 488847 2.4.1.19 6.8 - - 701849 2.4.1.19 6.8 - strain BC8 enzyme 701849 2.4.1.19 7 - - 488826, 488834, 488839, 488855, 702819, 735782, 735838, 756523 2.4.1.19 7 - alpha- and beta-cyclizing activity assay at 703068 2.4.1.19 7 - assay at 702108, 703802 2.4.1.19 7 - ATCC 21783 contains 3 isozymes, neutral, alkalic and acidic, possessing markedly different pH optima, 4.6, 7.0 and 9.5 488834 2.4.1.19 7 - ATCC 21783, purified enzyme, crude enzyme has 3 pH optima, 4.5-5.0, 7.0 and 8.0-9.0 488826 2.4.1.19 7 - ATCC 8514, IFO3490 488855 2.4.1.19 7 - enzyme immobilized on glyoxyl-agarose and free enzyme present similar pH/activities profiles, with two peaks at pH values of 5.5 and 7 and a minimum at pH 6.0-6.5 in both amylolytic and CGTase activities 672766 2.4.1.19 7 - in sodium phosphate buffer 758336 2.4.1.19 7 - presence of 1.5 M NaCl 719431 2.4.1.19 7 - starch degrading activity, mutant enzyme F283L 660462 2.4.1.19 7 8 pH optimum for gamma-cyclodextrin production 757274 2.4.1.19 7 10 strain AL-6 488836, 488855 2.4.1.19 7.2 - - 488855, 684523 2.4.1.19 7.4 - - 737302 2.4.1.19 7.5 - - 673390 2.4.1.19 7.5 - assay at 702826 2.4.1.19 7.5 8.5 var. alkalophilus ATCC 21783, second pH optimum at 4.5-4.7 488855 2.4.1.19 7.5 10.5 strain AL-6, cyclodextrin forming 488836 2.4.1.19 8 - - 488836, 756523 2.4.1.19 8 - and 6.0 718610 2.4.1.19 8 - beta-cyclodextrin production shows increment up to pH 8.0 but decreases at pH values higher than 9 657629 2.4.1.19 8 - strain AL-6, starch dextrinizing 488836 2.4.1.19 8 9 - 488861 2.4.1.19 8 9 ATCC 21783, crude enzyme, second pH optimum at 4.5-5.0 488826 2.4.1.19 8 9 strain BA-4229 488861 2.4.1.19 8 10 gamma-cyclodextrin production, reaction with 10% starch 657629 2.4.1.19 8.5 - - 658790, 757110 2.4.1.19 8.5 - assay at 688313, 701849 2.4.1.19 8.5 - strain B-3103 488861 2.4.1.19 8.5 - strain B4006 488861 2.4.1.19 8.6 - two maxima at pH 6.0 and pH 8.6 684450 2.4.1.19 9 - - 488831, 488837, 685718, 719615, 757109 2.4.1.19 9 - and 6.0 720955 2.4.1.19 9 - and a second optimum at pH 5.5 671395 2.4.1.19 9 - assay at 702109 2.4.1.19 9 - ATCC 21783, immobilized enzyme, acid enzyme activity disappeared 488831 2.4.1.19 9 - ATCC 39612/Ha3-3-2, starch dextrinizing activity, second pH optimum at 5.0 488832 2.4.1.19 9 - gamma-cyclodextrin production, reaction with 1% starch 657629 2.4.1.19 9 - pH optimum for beta-cyclodextrin production 757274 2.4.1.19 9 11 strain dependent 684530 2.4.1.19 9.5 - ATCC 21783 contains 3 isozymes, neutral, alkalic and acidic, possessing markedly different pH optima, 4.6, 7.0 and 9.5 488834 2.4.1.19 10 - - 488855, 758263 2.4.1.19 10 - AL-6 488855 2.4.1.19 10 - around, wild-type enzyme and mutant enzymes A223H, A223R and A223K, gamma-cyclodextrin-forming activity 674367 2.4.1.19 10.3 - var. alkalophilus, ATCC 21783 488855