2.4.1.10 additional information - pI: 4.7 488335 2.4.1.10 additional information - pI: 5.5 488345 2.4.1.10 4 4.6 45°C, optimal enzymatic activity is observed in this range, the influence of the buffering substance is not signifcant 684765 2.4.1.10 4 6 - 488312, 488331 2.4.1.10 4 6 recombinant enzyme 488331 2.4.1.10 5 - - 488345, 755924 2.4.1.10 5 - assay at 488343, 736130 2.4.1.10 5 - wild-type and mutant D309N 488340 2.4.1.10 5 7 - 488335 2.4.1.10 5.2 - - 706915 2.4.1.10 5.2 - assay at 488339, 701566 2.4.1.10 5.2 - free enzyme 701567 2.4.1.10 5.2 6 free and immobilized enzymes 702972 2.4.1.10 5.4 - - 671507 2.4.1.10 5.5 - - 488310, 736648, 756953 2.4.1.10 5.5 6 recombinant enzyme 735834 2.4.1.10 5.5 7 hydrolysis rate increases beyond pH 5.0 488332 2.4.1.10 5.6 - immobilized enzyme 701567 2.4.1.10 5.6 6 - 488321 2.4.1.10 5.75 - assay at 488333, 488334 2.4.1.10 5.8 6.6 sucrose hydrolysis 736891 2.4.1.10 5.9 - - 755877 2.4.1.10 6 - - 488308, 488309, 488313, 488329, 488330, 755827, 756158, 757042, 757356 2.4.1.10 6 - assay at 488311, 488341, 706683, 730222, 735888, 735890, 736849 2.4.1.10 6 - D-glucose production, recombinant enzyme 704960 2.4.1.10 6 - hydrolysis of sucrose to glucose and fructose 488319 2.4.1.10 6 - in 3.8 M phosphate buffer 488324 2.4.1.10 6 - recombinant enzyme 488326 2.4.1.10 6 - sucrose hydrolysis 736891 2.4.1.10 6 6.2 - 488321 2.4.1.10 6 6.6 sucrose hydrolysis 736891 2.4.1.10 6 7 - 678072 2.4.1.10 6 7 wild-type, 50 mM Sorensen’s phosphate buffer at 37°C, containing 500 mM sucrose using an enzyme concentration of 7.36 mg/l 678072 2.4.1.10 6.2 - levan synthesis 672335 2.4.1.10 6.5 - - 735894, 736295, 756945, 756954, 757280 2.4.1.10 6.5 - hydrolysis of sucrose to glucose and fructose 488314 2.4.1.10 6.5 - in 0.24 M phosphate buffer 488324 2.4.1.10 6.5 - in 50 mM sodium phosphate buffer 757507 2.4.1.10 6.5 - native and recombinant enzyme 737179 2.4.1.10 6.5 - transfructosylation and hydrolytic activity 735800 2.4.1.10 7 - - 705086 2.4.1.10 7 - assay at 488316, 488319, 488320 2.4.1.10 8 - levan formation, recombinant enzyme 704960 2.4.1.10 8.2 - - 757108