3.2.1.18 additional information - additional information kcat at pH 9.5, 37°C, relative to the reported kcat at pH 6.0, 37°C, with substrate 4-nitrophenyl alpha-D-N-acetylneurminide 655779 3.2.1.18 0.0004 - 4-methylumbelliferyl D-N-acetylneuraminyl-alpha-(2->3)-D-galactopyranoside pH 3.5, 37°C 717646 3.2.1.18 0.031 - 4-nitrophenyl alpha-sialoside mutant D59A, hydrolysis, pH 7.6, 25°C 696254 3.2.1.18 0.045 - 4-methylumbelliferyl alpha-D-N-acetylneuraminic acid mutant enzyme H275Y/I223V, at 37°C, pH not specified in the publication 752324 3.2.1.18 0.054 - 4-methylumbelliferyl alpha-D-N-acetylneuraminic acid mutant enzyme H275Y, at 37°C, pH not specified in the publication 752324 3.2.1.18 0.0743 - 4-methylumbelliferyl alpha-D-N-acetylneuraminic acid pH 3.5, 37°C 717646 3.2.1.18 0.1 - 4-methylumbelliferyl alpha-D-N-acetylneuraminic acid mutant enzyme H275Y/S247N, at 37°C, pH not specified in the publication 752324 3.2.1.18 0.17 - 4-methylumbelliferyl alpha-D-N-acetylneuraminic acid mutant enzyme S247N, at 37°C, pH not specified in the publication 752324 3.2.1.18 0.28 - 5-bromo-4-chloro-indolyl beta-D-galactopyranosyl-alpha-2,6-N-acetyl-neuraminic acid isoform Am0705, at pH 6.5 and 37°C 750235 3.2.1.18 0.6 - 4-methylumbelliferyl alpha-D-N-acetylneuraminic acid mutant enzyme I223V, at 37°C, pH not specified in the publication 752324