2.1.1.258 0.00003 - methylcobalamin mutant enzyme N199A, at pH 8.4 and 55°C 719846 2.1.1.258 0.005 - [Co(I) corrinoid Fe-S protein] mutant enzyme N199A, in 0.1 M potassium succinate, pH 5.0, 60 mM NaCl, at 25°C 719846 2.1.1.258 0.02 - [methyl-Co(III) corrinoid Fe-S protein] kcat below 0.02 s(-1), in 1 mM Tris-HCl (pH 7.6), at 25°C 718823 2.1.1.258 0.05 - methylcobalamin wild type enzyme, at pH 8.4 and 55°C 719846 2.1.1.258 1.1 - hydroxocobalamin in 1 mM Tris-HCl (pH 7.6), at 25°C 718823 2.1.1.258 1.1 - [Co(I) corrinoid Fe-S protein] wild type enzyme, in 0.1 M potassium succinate, pH 5.0, 60 mM NaCl, at 25°C 719846 2.1.1.258 1.5 - 5-methyltetrahydrofolate using hydroxocobalamin as cosubstrate, in 1 mM Tris-HCl (pH 7.6), at 25°C 718823 2.1.1.258 2.2 - methylcobalamin in 1 mM Tris-HCl (pH 7.6), at 25°C 718823 2.1.1.258 2.63 - Cobalamin 25°C, pH 5.1 728242 2.1.1.258 14.7 - 5-methyltetrahydrofolate in 50 mM MES, pH 5.1, at 25°C 717236