5.4.99.5	additional information	-	2-[5-Amino-2-(4-fluoro-phenyl)-6-oxo-6H-pyrimidin-1-yl]-N-(1-benzyl-2-oxo-2-thiazol-2-yl-ethyl)-acetamide	turnover of mutant H189N enzyme is lower than 0.0025 per second	390552	
5.4.99.5	additional information	-	additional information	CM spezific activity in Escherichia coli extracts: Control (M. Tuberculosis) Sp act (U/mg) 0.889 Cloned extract/control 1 AroQMt (Rv0948c) Sp act (U/mg) 127.38 Cloned extract/control 143.28 *AroQMt (Rv1885c) Sp act (U/mg) 94.23 Cloned extract/control 105.9. CM specific activity in Escherichia coli cells expressing either the AroQMt or the *AroQMt protein is determined to be, repectively, 143- and 106-fold higher than the enzyme activity obtained for Escherichia coli cells carrying the pET-23a(+) expression vector	674346	
5.4.99.5	additional information	-	additional information	R90Cit 10E4-fold decrease in the catalytic activity of kcat. R90K 10E4-fold decrease in the catalytic activity of kcat/Km is obtained	675611	
5.4.99.5	additional information	-	additional information	The kcat of the active complementations (position 7, 32, 35, 48, 81 and 85) is shown	672268	
5.4.99.5	additional information	-	additional information	turnover of mutant H189N enzyme is lower than 0.0025 per second	390552	
5.4.99.5	0.0003	-	chorismate	30°C, pH 7, mutant R90G	652061	
5.4.99.5	0.00983	-	chorismate	mutant enzyme Lys39Arg	3568	
5.4.99.5	0.042	-	chorismate	mutant enzyme Q88N, in PBS buffer (pH 7.5) at 20°C	690920	
5.4.99.5	0.0492	-	chorismate	mutant enzyme Lys39Asn	3568	
5.4.99.5	0.11	-	chorismate	mutant enzyme G86A	703449