5.4.99.5 additional information - 2-[5-Amino-2-(4-fluoro-phenyl)-6-oxo-6H-pyrimidin-1-yl]-N-(1-benzyl-2-oxo-2-thiazol-2-yl-ethyl)-acetamide turnover of mutant H189N enzyme is lower than 0.0025 per second 390552 5.4.99.5 0.0003 - chorismate 30°C, pH 7, mutant R90G 652061 5.4.99.5 0.00983 - chorismate mutant enzyme Lys39Arg 3568 5.4.99.5 0.042 - chorismate mutant enzyme Q88N, in PBS buffer (pH 7.5) at 20°C 690920 5.4.99.5 0.0492 - chorismate mutant enzyme Lys39Asn 3568 5.4.99.5 0.11 - chorismate mutant enzyme G86A 703449 5.4.99.5 0.123 - chorismate mutant enzyme Lys39Arg 3568 5.4.99.5 0.29 - chorismate 30°C, pH 7, mutant C88S/R90K 652061 5.4.99.5 0.32 - chorismate 30°C, pH 7, mutant C88K/R90S 652061 5.4.99.5 0.41 - chorismate mutant enzyme F77W, in PBS buffer (pH 7.5) at 20°C 690920