2.8.1.6 additional information - additional information BioB is active for only one turnover, during which the [2Fe-2S]2+ cluster is destroyed, one sulfide from the cluster is incorporated as the biotin thiophane sulfur, while Fe2+ ions and the remaining S2- ion are released from the protein 690617 2.8.1.6 0.0000005 - dethiobiotin pH 7.5, 30°C, cell free extract 645605 2.8.1.6 0.0000142 - dethiobiotin pH 7.5, 30°C, cell free extract 645605 2.8.1.6 13.9 - dethiobiotin pH 7.5, 37°C 645603 2.8.1.6 0.00055 - dethiobiotin pH 8, 37°C 645610 2.8.1.6 0.0016 - dethiobiotin pH 8.0, 37°C 762502