6.1.1.16 additional information - additional information tRNAGln mutant substrates 652821 6.1.1.16 additional information - additional information turnover numbers of mutant tRNA molecules 182 6.1.1.16 0.001 - L-cysteine mutant enzyme H40A/R42A, pH and temperature not specified in the publication 727889 6.1.1.16 0.005 - tRNACys aminoacylation reaction, recombinant C28S/C209S mutant enzyme 652909 6.1.1.16 0.007 - tRNACys aminoacylation reaction, recombinant W205Y mutant enzyme 652909 6.1.1.16 0.0093 - L-cysteine mutant enzyme H40A, pH and temperature not specified in the publication 727889 6.1.1.16 0.014 - tRNACys pH 7.5, 40°C, recombinant mutant D417A/E420A 672180 6.1.1.16 0.017 - L-Cysteinyl-tRNACys - 181 6.1.1.16 0.02 - L-cysteine mutant enzyme M294A/R427A, pH and temperature not specified in the publication 727889 6.1.1.16 0.027 - L-cysteine mutant enzyme R427A, pH and temperature not specified in the publication 727889 6.1.1.16 0.03 - tRNA3Cys value above, substrate tRNA3Cys isoacceptor 3 687743 6.1.1.16 0.03 - tRNACys mutant enzyme N351D, mutant tRNACys(G15-C48) 662934 6.1.1.16 0.03 - tRNACys pH 7.5, 40°C, recombinant mutant D435A/D436A 672180 6.1.1.16 0.036 - tRNACys mutant enzyme E354A, mutant tRNACys(G15-C48) 662934 6.1.1.16 0.05 - tRNA3CysU33A value above, substrate tRNA3Cys isoacceptor 3, containing a U33A mutation 687743 6.1.1.16 0.06 - tRNA2Cys value above, substrate tRNA2Cys isoacceptor 2 687743 6.1.1.16 0.06 - tRNACys pH 7.5, 40°C, recombinant mutant D239A/D240A 672180 6.1.1.16 0.074 - tRNACys mutant enzyme E354Q, mutant tRNACys(G15-C48) 662934 6.1.1.16 0.082 - L-cysteine mutant enzyme E354Q, pH and temperature not specified in the publication 727889 6.1.1.16 0.088 - L-cysteine mutant enzyme M294A/H297A, pH and temperature not specified in the publication 727889 6.1.1.16 0.09 - tRNA3CysG57A value above, substrate tRNA3Cys isoacceptor 3, containing a G57A mutation 687743 6.1.1.16 0.094 - tRNACys mutant enzyme N351D, wild-type tRNACys(G15-G48) 662934 6.1.1.16 0.1 - tRNACys mutant enzyme E354A, wild-type tRNACys(G15-G48) 662934 6.1.1.16 0.11 - tRNACys pH 7.5, 40°C, recombinant wild-type enzyme 672180 6.1.1.16 0.14 - L-cysteine mutant enzyme M294A, pH and temperature not specified in the publication 727889 6.1.1.16 0.14 - tRNA3CysC20U/U21C/A44U/C46A/A47G value above, substrate tRNA3Cys isoacceptor 3, containing a C20U/U21C/A44U/C46A/A47G mutation 687743 6.1.1.16 0.14 - tRNACys mutant enzyme E354Q, wild-type tRNACys(G15-G48) 662934 6.1.1.16 0.16 - L-cysteine mutant enzyme H297A, pH and temperature not specified in the publication 727889 6.1.1.16 0.16 - tRNA1CysA33U value above, substrate tRNA1Cys isoacceptor 1, containing a A33U mutation 687743 6.1.1.16 0.17 - tRNA1Cys value above, substrate tRNA1Cys isoacceptor 1 687743 6.1.1.16 0.18 - tRNA3CysC20U/U21C/A44U/C46A/A47G/G57A value above, substrate tRNA3Cys isoacceptor 3, containing a C20U/U21C/A44U/C46A/A47G/G57A mutation 687743 6.1.1.16 0.2 - tRNACys recombinant His-tagged deletion mutant, amino acid activation step 675435 6.1.1.16 0.28 - L-cysteine ATP-diphosphate exchange reaction, recombinant W205Y mutant enzyme 652909 6.1.1.16 0.3 - ATP ATP-diphosphate exchange reaction, recombinant W205Y mutant enzyme 652909 6.1.1.16 0.34 - ATP pH 8.0, mutant enzyme DELTA328-461, ATP-diphosphate exchange 661203 6.1.1.16 0.39 - tRNACys mutant enzyme N351A, mutant tRNACys(G15-C48) 662934 6.1.1.16 0.45 - tRNACys wild-type enzyme, mutant tRNACys(G15-C48) 662934 6.1.1.16 0.48 - ATP 30°C, pH 7.0, mutant enzyme V27E 662024 6.1.1.16 0.49 - L-cysteine mutant enzyme E354Q/R427A, pH and temperature not specified in the publication 727889 6.1.1.16 0.5 - tRNACys aminoacylation reaction, recombinant His-tagged Co2+-wild-type enzyme 652909 6.1.1.16 0.59 - tRNACys mutant enzyme N351A, wild-type tRNACys(G15-G48) 662934 6.1.1.16 0.68 - tRNACys 30°C, pH 7.0, mutant enzyme V27E 662024 6.1.1.16 0.86 - L-cysteine 30°C, pH 7.0, mutant enzyme V27E 662024 6.1.1.16 0.9 - tRNACys aminoacylation reaction, recombinant Zn2+-wild-type enzyme 652909 6.1.1.16 1.1 - L-cysteine mutant enzyme R42A, pH and temperature not specified in the publication 727889 6.1.1.16 1.2 - L-cysteine wild type enzyme, pH and temperature not specified in the publication 727889 6.1.1.16 2 - ATP pH 8.0, mutant enzyme DELTA288-461, ATP-diphosphate exchange 661203 6.1.1.16 2.2 - L-cysteine recombinant wild-type enzyme, pH 7.0, 37°C 653662 6.1.1.16 2.3 - tRNACys recombinant His-tagged wild-type full-length enzyme, amino acid activation step 675435 6.1.1.16 2.46 - tRNACys 37°C, wild-type enzyme 661203 6.1.1.16 2.46 - tRNACys wild-type enzyme, wild-type tRNACys(G15-G48) 662934 6.1.1.16 2.5 - L-cysteine steady-state 675395 6.1.1.16 2.5 - tRNACys aminoacylation reaction, recombinant His-tagged Zn2+-wild-type enzyme 652909 6.1.1.16 2.9 - tRNACys 30°C, pH 7.0, wild-type enzyme 662024 6.1.1.16 2.9 - tRNACys steady-state 675395 6.1.1.16 3 6 tRNACys pH 7.0, 37°C 652821 6.1.1.16 3.47 - tRNACys pH 7.0, 37°C 652821 6.1.1.16 4.4 - ATP 30°C, pH 7.0, wild-type enzyme 662024 6.1.1.16 4.8 - L-cysteine 30°C, pH 7.0, wild-type enzyme 662024 6.1.1.16 10.3 - L-cysteine recombinant His-tagged wild-type full-length enzyme, amino acid activation step 675435 6.1.1.16 18.1 - L-cysteine recombinant His-tagged deletion mutant, amino acid activation step 675435 6.1.1.16 22.6 - ATP pH 8.0, wild-type enzyme, ATP-diphosphate exchange 661203 6.1.1.16 35 - L-cysteine ATP-diphosphate exchange reaction, recombinant His-tagged Co2+-wild-type enzyme 652909 6.1.1.16 57 - ATP ATP-diphosphate exchange reaction, recombinant His-tagged Co2+-wild-type enzyme 652909 6.1.1.16 79 - L-cysteine ATP-diphosphate exchange reaction, recombinant Zn2+-wild-type enzyme 652909 6.1.1.16 91 - ATP ATP-diphosphate exchange reaction, recombinant Zn2+-wild-type enzyme 652909 6.1.1.16 100 - L-cysteine ATP-diphosphate exchange reaction, recombinant His-tagged Zn2+-wild-type enzyme 652909 6.1.1.16 142 - ATP ATP-diphosphate exchange reaction, recombinant His-tagged Zn2+-wild-type enzyme 652909