5.4.99.64 0.001 - isobutanoyl-CoA pH 6.6, 30°C, wild-type enzyme 736409 5.4.99.64 0.002 - (S)-3-hydroxybutanoyl-CoA mutant I90A, pH 6.6, 30°C 738677 5.4.99.64 0.002 - (S)-3-hydroxybutanoyl-CoA pH 6.6, 30°C, mutant enzyme I90A (mutation in subunit HcmA) 738677 5.4.99.64 0.003 - (R)-3-hydroxybutanoyl-CoA wild-type, pH 6.6, 30°C 738677 5.4.99.64 0.003 - (S)-3-hydroxybutanoyl-CoA mutant I90L, pH 6.6, 30°C 738677 5.4.99.64 0.0033 - (R)-3-hydroxybutanoyl-CoA pH 6.6, 30°C, wild-type enzyme 736409, 738677 5.4.99.64 0.0033 - butanoyl-CoA pH 6.6, 30°C, wild-type enzyme 736409 5.4.99.64 0.0035 - (R)-3-hydroxybutanoyl-CoA pH 6.6, 30°C, mutant enzyme I90A (mutation in subunit HcmA) 738677 5.4.99.64 0.0035 - (S)-3-hydroxybutanoyl-CoA pH 6.6, 30°C, mutant enzyme I90L (mutation in subunit HcmA) 738677 5.4.99.64 0.004 - (R)-3-hydroxybutanoyl-CoA mutant I90A, pH 6.6, 30°C 738677 5.4.99.64 0.0067 - 2-hydroxy-2-methylpropanoyl-CoA pH 6.6, 30°C, mutant enzyme I90L (mutation in subunit HcmA) 738677 5.4.99.64 0.007 - 2-hydroxy-2-methylpropanoyl-CoA mutant I90L, pH 6.6, 30°C 738677 5.4.99.64 0.009 - isovaleryl-CoA pH 6.6, 30°C, mutant enzyme I90A (mutation in subunit HcmA) 738677 5.4.99.64 0.0093 - isovaleryl-CoA pH 6.6, 30°C, mutant enzyme I90V (mutation in subunit HcmA) 738677 5.4.99.64 0.011 - isovaleryl-CoA pH 6.6, 30°C, wild-type enzyme 738677 5.4.99.64 0.013 - 2,2-dimethylpropanoyl-CoA pH 6.6, 30°C, mutant enzyme I90A (mutation in subunit HcmA) 738677 5.4.99.64 0.016 - 2,2-dimethylpropanoyl-CoA pH 6.6, 30°C, wild-type enzyme 738677 5.4.99.64 0.017 - 2,2-dimethylpropanoyl-CoA pH 6.6, 30°C, mutant enzyme I90V (mutation in subunit HcmA) 738677 5.4.99.64 0.02 - 2-hydroxy-2-methylpropanoyl-CoA mutant I90V, pH 6.6, 30°C 738677 5.4.99.64 0.02 - 2-hydroxy-2-methylpropanoyl-CoA pH 6.6, 30°C, mutant enzyme I90V 736409 5.4.99.64 0.02 - 2-hydroxy-2-methylpropanoyl-CoA pH 6.6, 30°C, mutant enzyme I90V (mutation in subunit HcmA) 738677 5.4.99.64 0.0245 - 2-hydroxy-2-methylpropanoyl-CoA pH 6.6, 30°C, mutant enzyme I90A (mutation in subunit HcmA) 738677 5.4.99.64 0.025 - 2-hydroxy-2-methylpropanoyl-CoA mutant I90A, pH 6.6, 30°C 738677 5.4.99.64 0.0255 - (S)-3-hydroxybutanoyl-CoA pH 6.6, 30°C, mutant enzyme D117A (mutation in subunit HcmA) 738677 5.4.99.64 0.026 - (S)-3-hydroxybutanoyl-CoA mutant D117A, pH 6.6, 30°C 738677 5.4.99.64 0.027 - (R)-3-hydroxybutanoyl-CoA mutant D117A, pH 6.6, 30°C 738677 5.4.99.64 0.027 - (R)-3-hydroxybutanoyl-CoA pH 6.6, 30°C, mutant enzyme D117A (mutation in subunit HcmA) 738677 5.4.99.64 0.033 - (S)-3-hydroxybutanoyl-CoA mutant I90V, pH 6.6, 30°C 738677 5.4.99.64 0.033 - (S)-3-hydroxybutanoyl-CoA pH 6.6, 30°C, mutant enzyme I90V 736409 5.4.99.64 0.033 - (S)-3-hydroxybutanoyl-CoA pH 6.6, 30°C, mutant enzyme I90V (mutation in subunit HcmA) 738677 5.4.99.64 0.044 - 2-hydroxy-2-methylpropanoyl-CoA mutant D117A, pH 6.6, 30°C 738677 5.4.99.64 0.044 - 2-hydroxy-2-methylpropanoyl-CoA pH 6.6, 30°C, mutant enzyme D117A (mutation in subunit HcmA) 738677 5.4.99.64 0.045 - 2-hydroxy-2-methylpropanoyl-CoA mutant D117V, pH 6.6, 30°C 738677 5.4.99.64 0.045 - 2-hydroxy-2-methylpropanoyl-CoA pH 6.6, 30°C, mutant enzyme D117V (mutation in subunit HcmA) 738677 5.4.99.64 0.05 - 2-hydroxy-2-methylpropanoyl-CoA pH 6.6, 30°C, wild-type enzyme 736409, 738677 5.4.99.64 0.05 - 2-hydroxy-2-methylpropanoyl-CoA wild-type, pH 6.6, 30°C 738677 5.4.99.64 0.062 - (S)-3-hydroxybutanoyl-CoA mutant D117V, pH 6.6, 30°C 738677 5.4.99.64 0.062 - (S)-3-hydroxybutanoyl-CoA pH 6.6, 30°C, mutant enzyme D117V (mutation in subunit HcmA) 738677 5.4.99.64 0.14 - 2,2-dimethylpropanoyl-CoA pH 6.6, 30°C, mutant enzyme D117V (mutation in subunit HcmA) 738677 5.4.99.64 0.17 - (R)-3-hydroxybutanoyl-CoA pH 6.6, 30°C, mutant enzyme D117V (mutation in subunit HcmA) 738677 5.4.99.64 0.173 - (R)-3-hydroxybutanoyl-CoA mutant D117V, pH 6.6, 30°C 738677 5.4.99.64 0.2 - (S)-3-hydroxybutanoyl-CoA pH 6.6, 30°C, wild-type enzyme 736409, 738677 5.4.99.64 0.2 - (S)-3-hydroxybutanoyl-CoA wild-type, pH 6.6, 30°C 738677 5.4.99.64 1.6 - (S)-3-hydroxybutanoyl-CoA pH 7.8, 55°C 737504 5.4.99.64 3.7 - 2-hydroxyisobutanoyl-CoA pH 7.8, 55°C 737504 5.4.99.64 4.9 - (R)-3-hydroxybutanoyl-CoA pH 7.8, 55°C 737504