5.4.99.5 26 - chorismate - 662365 5.4.99.5 50 - chorismate - 3562 5.4.99.5 60 - chorismate +/-4, substrate chorismate 674312 5.4.99.5 5.7 - chorismate 30°C 649832 5.4.99.5 0.32 - chorismate 30°C, pH 7, mutant C88K/R90S 652061 5.4.99.5 0.29 - chorismate 30°C, pH 7, mutant C88S/R90K 652061 5.4.99.5 0.0003 - chorismate 30°C, pH 7, mutant R90G 652061 5.4.99.5 46 - chorismate 30°C, pH 7, wild-type 652061 5.4.99.5 7.2 - chorismate 30°C, pH 7.2, mutant H131A 390552 5.4.99.5 10 - chorismate 30°C, pH 7.2, mutant H153N 390552 5.4.99.5 16 - chorismate 30°C, pH 7.2, mutant H197N 390552 5.4.99.5 2.3 - chorismate 30°C, pH 7.2, mutant H239N 390552 5.4.99.5 4.8 - chorismate 30°C, pH 7.2, mutant H245N 390552 5.4.99.5 8 - chorismate 30°C, pH 7.2, mutant H257A 390552 5.4.99.5 15 - chorismate 30°C, pH 7.2, mutant H265A 390552 5.4.99.5 6 - chorismate 30°C, pH 7.2, mutant H347N 390552 5.4.99.5 27 - chorismate 30°C, pH 7.2, wild-type 390552 5.4.99.5 26 - chorismate 30°C, pH 7.5, mutant DELTA117-127, with 3.8 mM substrate and DELTA 118-127/R116L/P117T, with 3.9 mM substrate 649938 5.4.99.5 24 - chorismate 30°C, pH 7.5, mutant DELTA118-127, with 3.4 mM substrate 649938 5.4.99.5 22 - chorismate 30°C, pH 7.5, mutant DELTA118-127, with 5.8 mM substrate 649938 5.4.99.5 30 - chorismate 30°C, pH 7.5, mutant DELTA118-127/K111N/A112S/V113N, with 4 mM substrate 649938 5.4.99.5 23 - chorismate 30°C, pH 7.5, mutant DELTA119-127/D118N, with 5.8 mM substrate 649938 5.4.99.5 47 - chorismate 30°C, pH 7.5, wild-type, with 4 mM substrate 649938 5.4.99.5 82 - chorismate 30°C, pH 7.6 651936 5.4.99.5 92 - chorismate 30°C, pH 7.6, in presence of 0.005 mM tryptophan 651936 5.4.99.5 367 - chorismate 37°C 651660 5.4.99.5 23.8 - chorismate 37°C, in presence of 0.1 mM tyrosine 651660 5.4.99.5 89.3 - chorismate 37°C, in presence of 0.1 mM tyrosine 651660 5.4.99.5 148900 - chorismate 37°C, pH 7.8, chorismate mutase 650528 5.4.99.5 149000 - chorismate 37°C, pH 7.8, chorismate mutase 650528 5.4.99.5 365600 - chorismate 37°C, pH 7.8, DELTA102-285 650528 5.4.99.5 366000 - chorismate 37°C, pH 7.8, DELTA102-285 650528 5.4.99.5 234000 - chorismate 37°C, pH 7.8, DELTA102-285 in presence of 0.05 mM phenylalanine 650528 5.4.99.5 234100 - chorismate 37°C, pH 7.8, DELTA102-285 in presence of 0.05 mM phenylalanine 650528 5.4.99.5 253000 - chorismate 37°C, pH 7.8, DELTA102-285 in presence of 0.5 mM phenylalanine 650528 5.4.99.5 253400 - chorismate 37°C, pH 7.8, DELTA102-285 in presence of 0.5 mM phenylalanine 650528 5.4.99.5 257900 - chorismate 37°C, pH 7.8, DELTA102-285 in presence of 2 mM phenylalanine 650528 5.4.99.5 258000 - chorismate 37°C, pH 7.8, DELTA102-285 in presence of 2 mM phenylalanine 650528 5.4.99.5 94100 - prephenate 37°C, pH 7.8, in presence of P-protein 650528 5.4.99.5 94140 - prephenate 37°C, pH 7.8, in presence of P-protein 650528 5.4.99.5 140500 - chorismate 37°C, pH 7.8, in presence of P-protein 650528 5.4.99.5 141000 - chorismate 37°C, pH 7.8, in presence of P-protein 650528 5.4.99.5 52 - chorismate 70°C, pH 7.6 649515 5.4.99.5 1.05 - chorismate at 37°C 705570 5.4.99.5 5.5 - chorismate at 37°C and pH 7.5 692314 5.4.99.5 70 - chorismate at 37°C and pH 7.5 692314 5.4.99.5 64 - chorismate chorismate mutase domain of P-protein 651595 5.4.99.5 additional information - additional information CM spezific activity in Escherichia coli extracts: Control (M. Tuberculosis) Sp act (U/mg) 0.889 Cloned extract/control 1 AroQMt (Rv0948c) Sp act (U/mg) 127.38 Cloned extract/control 143.28 *AroQMt (Rv1885c) Sp act (U/mg) 94.23 Cloned extract/control 105.9. CM specific activity in Escherichia coli cells expressing either the AroQMt or the *AroQMt protein is determined to be, repectively, 143- and 106-fold higher than the enzyme activity obtained for Escherichia coli cells carrying the pET-23a(+) expression vector 674346 5.4.99.5 44.3 - chorismate genetically engineered enzyme containg the amino acid residues 1-285 3570 5.4.99.5 40.7 - chorismate genetically engineered enzyme containg the amino acid residues 1-300 3570 5.4.99.5 56 - chorismate leaderless MtCM with C-terminal His tag, at 30°C and pH 7.5 661703 5.4.99.5 45.13 - chorismate mutant A32S 663283 5.4.99.5 2 8 chorismate mutant DELTA117-127 651595 5.4.99.5 1.3 - chorismate mutant enzyme D48G, in PBS buffer (pH 7.5) at 20°C 690920 5.4.99.5 0.41 - chorismate mutant enzyme F77W, in PBS buffer (pH 7.5) at 20°C 690920 5.4.99.5 0.11 - chorismate mutant enzyme G86A 703449 5.4.99.5 14 - chorismate mutant enzyme Glu23Ala, activated by 0.01 mM Trp 3572 5.4.99.5 625 - chorismate mutant enzyme Glu23Asp, activated by 0.01 mM Trp 3572 5.4.99.5 171 - chorismate mutant enzyme Glu23Gln, activated by 0.01 mM Trp 3572 5.4.99.5 535 - chorismate mutant enzyme Ile225Thr/Thr226Ile, activated by 0.01 mM Trp 3572 5.4.99.5 0.00983 - chorismate mutant enzyme Lys39Arg 3568 5.4.99.5 0.123 - chorismate mutant enzyme Lys39Arg 3568 5.4.99.5 0.0492 - chorismate mutant enzyme Lys39Asn 3568 5.4.99.5 0.042 - chorismate mutant enzyme Q88N, in PBS buffer (pH 7.5) at 20°C 690920 5.4.99.5 2.6 - chorismate mutant enzyme R51Q, in PBS buffer (pH 7.5) at 20°C 690920 5.4.99.5 351 - chorismate mutant enzyme Thr226Ile, activated by 0.01 mM Trp 3572 5.4.99.5 252 - chorismate mutant enzyme Tyr234Ala, activated by 0.01 mM Trp 3572 5.4.99.5 44 - chorismate mutant enzyme Tyr234Glu, activated by 0.01 mM Trp 3572 5.4.99.5 565 - chorismate mutant enzyme Tyr234Phe, activated by 0.01 mM Trp 3572 5.4.99.5 74 - chorismate mutant enzyme Tyr234Ser, activated by 0.01 mM Trp 3572 5.4.99.5 0.5 - chorismate mutant enzyme V35A, in PBS buffer (pH 7.5) at 20°C 690920 5.4.99.5 33.4 - chorismate mutant I81L/V85I 663283 5.4.99.5 36.55 - chorismate mutant L7I 663283 5.4.99.5 50.77 - chorismate mutant V35I 663283 5.4.99.5 60 - chorismate pH 7.0, 37°C 747259 5.4.99.5 46 - chorismate pH 7.5, 37°C, recombinant AroH 749355 5.4.99.5 0.78 - chorismate pH 7.5, 37°C, recombinant BsCM_2 749355 5.4.99.5 13 - chorismate pH 8.0, temperature not specified in the publication, recombinant enzyme 747032 5.4.99.5 15 - chorismate pH 8.0, temperature not specified in the publication, recombinant enzyme 747032 5.4.99.5 16 - chorismate pH 8.0, temperature not specified in the publication, recombinant enzyme 747032 5.4.99.5 18.8 - chorismate pH 8.0, temperature not specified in the publication, recombinant enzyme 747032 5.4.99.5 19.5 - chorismate pH 8.0, temperature not specified in the publication, recombinant enzyme 747032 5.4.99.5 20.7 - chorismate pH 8.0, temperature not specified in the publication, recombinant enzyme 747032 5.4.99.5 22.8 - chorismate pH 8.0, temperature not specified in the publication, recombinant enzyme 747032 5.4.99.5 39 - chorismate pH 8.0, temperature not specified in the publication, recombinant enzyme 747032 5.4.99.5 additional information - additional information R90Cit 10E4-fold decrease in the catalytic activity of kcat. R90K 10E4-fold decrease in the catalytic activity of kcat/Km is obtained 675611 5.4.99.5 13 - chorismate recombinant enzyme, pH 8.0, temperature not specified in the publication 748178 5.4.99.5 16.1 - chorismate recombinant enzyme, pH 8.0, temperature not specified in the publication 748178 5.4.99.5 38.7 - chorismate recombinant enzyme, pH 8.0, temperature not specified in the publication 748178 5.4.99.5 additional information - additional information The kcat of the active complementations (position 7, 32, 35, 48, 81 and 85) is shown 672268 5.4.99.5 additional information - 2-[5-Amino-2-(4-fluoro-phenyl)-6-oxo-6H-pyrimidin-1-yl]-N-(1-benzyl-2-oxo-2-thiazol-2-yl-ethyl)-acetamide turnover of mutant H189N enzyme is lower than 0.0025 per second 390552 5.4.99.5 additional information - additional information turnover of mutant H189N enzyme is lower than 0.0025 per second 390552 5.4.99.5 50 - chorismate untagged enzyme, at 30°C and pH 7.5 661703 5.4.99.5 38.87 - chorismate wild type 663283 5.4.99.5 2 - chorismate wild type enzyme 703449 5.4.99.5 3 - chorismate wild type enzyme, in PBS buffer (pH 7.5) at 20°C 690920 5.4.99.5 41 - chorismate wild-type 651595 5.4.99.5 39 - chorismate wild-type enzyme 3570 5.4.99.5 41.4 - chorismate wild-type enzyme 3568 5.4.99.5 361 - chorismate wild-type enzyme, activated by 0.01 mM Trp 3572