5.3.1.9 0.0003 - D-fructose 6-phosphate 50°C, pH 7.5, mutant enzyme E98V 651319 5.3.1.9 0.021 - D-fructose 6-phosphate 50°C, pH 7.5, mutant enzyme H89A 651319 5.3.1.9 0.04 - D-glucose 6-phosphate kcat below 0.04 s-1,mutant enzyme K362A, at 21°C in 20 mM HEPES buffer (pH 7.5) 726971 5.3.1.9 0.04 - D-glucose 6-phosphate kcat below 0.04 s-1mutant enzyme H389L, at 21°C in 20 mM HEPES buffer (pH 7.5) 726971 5.3.1.9 0.06 - D-fructose 6-phosphate pH 7.4, 70°C, mutant enzyme E93D 722225 5.3.1.9 0.07 - D-fructose 6-phosphate pH 7.4, 70°C, mutant enzyme G79L 722225 5.3.1.9 0.22 - D-fructose 6-phosphate pH 7.4, 70°C, mutant enzyme Y95F 722225 5.3.1.9 0.3 - D-glucose 6-phosphate pH 7.5, 30°C, recombinant mutant S278L 702456 5.3.1.9 0.32 - D-fructose 6-phosphate pH 7.4, 70°C, mutant enzyme G79A 722225 5.3.1.9 0.34 - D-fructose 6-phosphate 50°C, pH 7.5, mutant enzyme H137A 651319 5.3.1.9 0.42 - D-fructose 6-phosphate pH 7.4, 70°C, mutant enzyme H80D 722225 5.3.1.9 0.43 - D-fructose 6-phosphate pH 7.4, 70°C, mutant enzyme H80A 722225 5.3.1.9 0.5 - D-fructose 6-phosphate 50°C, pH 7.5, mutant enzyme H91A 651319 5.3.1.9 0.6 - D-fructose 6-phosphate pH 7.5, 30°C, recombinant mutant S278L 702456 5.3.1.9 0.6 - D-glucose 6-phosphate mutant enzyme H100L, at 21°C in 20 mM HEPES buffer (pH 7.5) 726971 5.3.1.9 0.68 - D-fructose 6-phosphate pH 7.4, 70°C, mutant enzyme Y95K 722225 5.3.1.9 1.3 - D-glucose 6-phosphate mutant enzyme D511N, at 21°C in 20 mM HEPES buffer (pH 7.5) 726971 5.3.1.9 1.6 - D-glucose 6-phosphate mutant enzyme E495Q, at 21°C in 20 mM HEPES buffer (pH 7.5) 726971 5.3.1.9 1.9 - D-fructose 6-phosphate pH 7.4, 70°C, mutant enzyme T63A 722225 5.3.1.9 4 5 D-glucose 6-phosphate mutant enzyme Q388A, at 21°C in 20 mM HEPES buffer (pH 7.5) 726971 5.3.1.9 5 - D-fructose 6-phosphate at pH 6.0 and 37°C 728836 5.3.1.9 8 - D-fructose 6-phosphate pH 7.4, 70°C, mutant enzyme H82A 722225 5.3.1.9 8.4 - D-fructose 6-phosphate 50°C 681378 5.3.1.9 11.6 - D-fructose 6-phosphate pH 7.4, 70°C, mutant enzyme Y160F 722225 5.3.1.9 15 - D-fructose 6-phosphate pH 7.5, 30°C, recombinant mutant L339P 702456 5.3.1.9 17 - D-glucose 6-phosphate pH 7.5, 30°C, recombinant mutant L487F 702456 5.3.1.9 18 - D-fructose 6-phosphate pH 8.0 704132 5.3.1.9 19 - D-fructose 6-phosphate pH 7.5, 30°C, recombinant mutant L487F 702456 5.3.1.9 21 - D-glucose 6-phosphate mutant enzyme H396L, at 21°C in 20 mM HEPES buffer (pH 7.5) 726971 5.3.1.9 26 - D-fructose 6-phosphate 50°C, pH 7.5, wild-type enzyme 651319 5.3.1.9 30.8 - D-fructose 6-phosphate pH 7.4, 70°C, mutant enzyme H136A 722225 5.3.1.9 31 - D-glucose 6-phosphate pH 7.5, 30°C, recombinant mutant L339P 702456 5.3.1.9 32.4 - D-fructose 6-phosphate pH 7.4, 70°C, wild-type enzyme 722225 5.3.1.9 42 - D-fructose 6-phosphate pH 7.5, 30°C, recombinant mutant R347H 702456 5.3.1.9 42 - D-glucose 6-phosphate 50°C, pH 7.5, wild-type enzyme 651319 5.3.1.9 61 - D-glucose 6-phosphate pH 7.5, 30°C, recombinant mutant A300P 702456 5.3.1.9 63 - D-glucose 6-phosphate pH 7.5, 30°C, recombinant mutant R347H 702456 5.3.1.9 75 - D-fructose 6-phosphate pH 7.5, 30°C, recombinant mutant T375R 702456 5.3.1.9 76 - D-fructose 6-phosphate pH 7.5, 30°C, recombinant mutant I525T 702456 5.3.1.9 80 - D-glucose 6-phosphate pH 7.5, 30°C, recombinant mutant T375R 702456 5.3.1.9 93 - D-fructose 6-phosphate pH 7.5, 30°C, recombinant mutant R75G 702456 5.3.1.9 100 - D-fructose 6-phosphate pH 7.5, 30°C, recombinant mutant A300P 702456 5.3.1.9 110 - D-glucose 6-phosphate pH 7.5, 30°C, recombinant mutant I525T 702456 5.3.1.9 120 - D-glucose 6-phosphate pH 7.5, 30°C, recombinant mutant R83W 702456 5.3.1.9 130 - D-glucose 6-phosphate pH 7.5, 30°C, recombinant mutant R75G 702456 5.3.1.9 140 - D-glucose 6-phosphate pH 7.5, 30°C, recombinant mutant V101M 702456 5.3.1.9 150 - D-fructose 6-phosphate pH 7.5, 30°C, recombinant mutant R347C 702456 5.3.1.9 160 - D-fructose 6-phosphate pH 7.5, 30°C, recombinant mutant V101M 702456 5.3.1.9 190 - D-fructose 6-phosphate pH 7.5, 30°C, recombinant mutant R472H 702456 5.3.1.9 190 - D-fructose 6-phosphate pH 7.5, 30°C, recombinant mutant R83W 702456 5.3.1.9 240 - D-glucose 6-phosphate mutant enzyme Y274F, at 21°C in 20 mM HEPES buffer (pH 7.5) 726971 5.3.1.9 260 - D-glucose 6-phosphate pH 7.5, 30°C, recombinant mutant R472H 702456 5.3.1.9 300 - D-fructose 6-phosphate pH 7.5, 30°C, recombinant mutant T195I 702456 5.3.1.9 340 - D-glucose 6-phosphate mutant enzyme Y341F, at 21°C in 20 mM HEPES buffer (pH 7.5) 726971 5.3.1.9 350 - D-glucose 6-phosphate pH 7.5, 30°C, recombinant mutant R347C 702456 5.3.1.9 360 - D-glucose 6-phosphate mutant enzyme S185A, at 21°C in 20 mM HEPES buffer (pH 7.5) 726971 5.3.1.9 380 - D-glucose 6-phosphate pH 7.5, 30°C, recombinant mutant T195I 702456 5.3.1.9 420 - D-fructose 6-phosphate pH 7.5, 30°C, recombinant mutant E495K 702456 5.3.1.9 470 - D-glucose 6-phosphate mutant enzyme N154Q, at 21°C in 20 mM HEPES buffer (pH 7.5) 726971 5.3.1.9 470 - D-glucose 6-phosphate mutant enzyme N386A, at 21°C in 20 mM HEPES buffer (pH 7.5) 726971 5.3.1.9 475.5 - L-talose pH 7.0, 95°C 701842 5.3.1.9 500 - D-glucose 6-phosphate wild type enzyme, at 21°C in 20 mM HEPES buffer (pH 7.5) 726971 5.3.1.9 650 - D-fructose 6-phosphate pH 7.5, 30°C, recombinant wild-type enzyme 702456 5.3.1.9 729 - D-glucose 6-phosphate cellulose-binding module-phosphoglucose isomerase, in 100 mM HEPES, 10 mM Mg2+ and 0.5 mM Mn2+, pH 7.5, 37°C 714592 5.3.1.9 750 - D-glucose 6-phosphate pH 7.5, 30°C, recombinant mutant E495K 702456 5.3.1.9 929 - D-glucose 6-phosphate free enzyme, in 100 mM HEPES, 10 mM Mg2+ and 0.5 mM Mn2+, pH 7.5, 37°C 714592 5.3.1.9 946 - D-glucose 6-phosphate avicel-cellulose-binding module-phosphoglucose isomerase, in 100 mM HEPES, 10 mM Mg2+ and 0.5 mM Mn2+, pH 7.5, 37°C 714592 5.3.1.9 1000 - D-glucose 6-phosphate pH 7.5, 30°C, recombinant wild-type enzyme 702456 5.3.1.9 1091 - D-glucose 6-phosphate immobilized-cellulose-binding module-phosphoglucose isomerase, in 100 mM HEPES, 10 mM Mg2+ and 0.5 mM Mn2+, pH 7.5, 37°C 714592 5.3.1.9 2009 - D-glucose 6-phosphate avicel-cellulose-binding module-phosphoglucose isomerase, in 100 mM HEPES, 10 mM Mg2+ and 0.5 mM Mn2+, pH 7.5, 60°C 714592 5.3.1.9 2198 - D-glucose 6-phosphate immobilized-cellulose-binding module-phosphoglucose isomerase, in 100 mM HEPES, 10 mM Mg2+ and 0.5 mM Mn2+, pH 7.5, 60°C 714592 5.3.1.9 2433 - D-glucose 6-phosphate cellulose-binding module-phosphoglucose isomerase, in 100 mM HEPES, 10 mM Mg2+ and 0.5 mM Mn2+, pH 7.5, 60°C 714592 5.3.1.9 2765 - D-glucose 6-phosphate free enzyme, in 100 mM HEPES, 10 mM Mg2+ and 0.5 mM Mn2+, pH 7.5, 60°C 714592 5.3.1.9 3330 - fructose 6-phosphate isomerase a 2830