5.1.1.3	additional information	-	additional information	mutant V149A has about a 2fold higher kcat than wild-type RacE2 (67/sec versus 38/sec). In the reverse reaction, V149A has a higher kcat than RacE2 (4.9/sec versus 1.6/sec, respectively)	675451	
5.1.1.3	0.00034	-	D-glutamate	pH 8.5, mutant enzyme D7S	653305	
5.1.1.3	0.0023	-	L-glutamate	pH 8.5, mutant enzyme E147N	653305	
5.1.1.3	0.0047	-	L-glutamate	pH 8.5, mutant enzyme E147N	653305	
5.1.1.3	0.017	-	L-glutamate	recombinant His-tagged enzyme, pH 8.0, 37°C	759787	
5.1.1.3	0.024	-	L-glutamate	recombinant His-tagged enzyme, pH 8.0, 37°C, bicine buffer	759787	
5.1.1.3	0.025	-	D-glutamate	recombinant enzyme, pH 8.0, 37°C	749098	
5.1.1.3	0.037	-	L-glutamate	pH 8.5, mutant enzyme D7S	653305	
5.1.1.3	0.045	-	L-glutamate	pH 8, 30°C, mutant enzyme H186N	650067	
5.1.1.3	0.056	-	D-glutamate	pH 8.5, wild-type enzyme	653305	
5.1.1.3	0.063	-	D-glutamate	pH 8, 30°C, mutant enzyme D10N	650067	
5.1.1.3	0.065	-	D-glutamate	deletion mutant A75T	676152	
5.1.1.3	0.068	-	L-glutamate	pH 8, 30°C, mutant enzyme D10N	650067	
5.1.1.3	0.08	-	D-glutamate	deletion mutant E151T	676152	
5.1.1.3	0.093	-	D-glutamate	pH 8, 30°C, mutant enzyme H186N	650067	
5.1.1.3	0.2	-	D-glutamate	-	676152	
5.1.1.3	0.21	-	L-glutamate	pH 8.5, wild-type enzyme	653305	
5.1.1.3	0.41	-	D-glutamate	-	676152	
5.1.1.3	0.41	-	L-glutamate	-	676152	
5.1.1.3	0.43	-	D-glutamate	mutant T76A, at 25°C in 50 mM Tris-HCl (pH 8.0), 5 mM NAD+, 0.5 mM iodonitrotetrazolium chloride, 2.5 mM ADP, 20 units of L-glutamate dehydrogenase, 2 units of diaphorase and D-glutamate	704192	
5.1.1.3	0.49	-	D-glutamate	-	675453	
5.1.1.3	0.56	-	D-glutamate	-	676152	
5.1.1.3	1	-	L-glutamate	recombinant His-tagged enzyme, pH 8.0, 70°C, bicine buffer	759787	
5.1.1.3	1.05	-	L-glutamate	-	676152	
5.1.1.3	1.3	-	D-glutamate	wild type enzyme, at 25°C in 50 mM Tris-HCl, pH 8.0, 5 mM NAD+, 0.5 mM iodonitrotetrazolium chloride, 2.5 mM ADP, 20 units of L-glutamate dehydrogenase, 2 units of diaphorase and D-glutamate	704192	
5.1.1.3	1.3	-	L-glutamate	wild-type enzyme, pH and temperature not specified in the publication	746586	
5.1.1.3	1.6	-	D-glutamate	-	675451	
5.1.1.3	1.63	-	L-glutamate	-	675453	
5.1.1.3	1.78	-	L-glutamate	deletion mutant A75T	676152	
5.1.1.3	1.8	-	D-glutamate	-	674336	
5.1.1.3	2	-	D-glutamate	-	674336	
5.1.1.3	2.26	-	L-glutamate	deletion mutant E151T	676152	
5.1.1.3	2.4	-	L-2-aminoadipic acid	His-tagged recombinant FnGR, in potassium phosphate buffer (10 mM, pH 8.0)	701927	
5.1.1.3	3.3	-	D-glutamate	-	691765	
5.1.1.3	3.4	-	L-glutamate	-	676152	
5.1.1.3	3.9	-	D-glutamate	-	675451	
5.1.1.3	6.8	-	L-glutamate	pH 8.0, 30°C	660592	
5.1.1.3	8.3	-	D-glutamate	His-tagged recombinant BsGR, in potassium phosphate buffer (10 mM, pH 8.0)	701927	
5.1.1.3	8.5	-	L-glutamate	-	676152	
5.1.1.3	11.73	-	D-glutamate	-	676152	
5.1.1.3	12	-	L-glutamate	-	674336	
5.1.1.3	12.16	-	L-glutamate	-	676152	
5.1.1.3	12.6	-	D-glutamate	pH 7.6, 37°C	746766	
5.1.1.3	13.8	-	L-glutamate	His-tagged recombinant FnGR, in potassium phosphate buffer (10 mM, pH 8.0)	701927	
5.1.1.3	15	-	D-glutamate	-	676152	
5.1.1.3	15	-	D-glutamate	His-tagged recombinant BsGR mutant V149A, in potassium phosphate buffer (10 mM, pH 8.0)	701927	
5.1.1.3	17.4	-	L-glutamate	recombinant FnGR, in potassium phosphate buffer (10 mM, pH 8.0)	701927	
5.1.1.3	17.7	-	L-glutamate	-	675451	
5.1.1.3	18	-	L-glutamate	-	674336	
5.1.1.3	20	-	L-glutamate	pH 8, 30°C, mutant enzyme D36N	650067	
5.1.1.3	22	-	D-glutamate	His-tagged recombinant FnGR, in potassium phosphate buffer (10 mM, pH 8.0)	701927	
5.1.1.3	22	-	D-glutamate	pH 8, 30°C, mutant enzyme D36N and H152Q	650067	
5.1.1.3	25	-	L-glutamate	-	676152	
5.1.1.3	26	-	D-glutamate	recombinant FnGR, in potassium phosphate buffer (10 mM, pH 8.0)	701927	
5.1.1.3	36.66	-	L-glutamate	-	676152	
5.1.1.3	38	-	L-glutamate	-	675451	
5.1.1.3	38	-	L-glutamate	pH 8, 30°C, mutant enzyme H152Q	650067	
5.1.1.3	43.33	-	D-glutamate	-	676152	
5.1.1.3	63	-	L-glutamate	His-tagged recombinant BsGR, in potassium phosphate buffer (10 mM, pH 8.0)	701927	
5.1.1.3	68	-	D-glutamate	pH 8, 30°C, wild-type enzyme	650067	
5.1.1.3	69	-	D-Glu	-	2104	
5.1.1.3	69	-	L-glutamate	pH 8, 30°C, wild-type enzyme	650067	
5.1.1.3	76	-	L-Glu	-	2104	
5.1.1.3	76	-	L-glutamate	His-tagged recombinant BsGR mutant V149A, in potassium phosphate buffer (10 mM, pH 8.0)	701927	
5.1.1.3	87	-	L-glutamate	wild type enzyme, 50 mM boric acid, 100 mM KCl, 0.2 mM dithiothreitol, pH 8.0, at 25°C in the presence of 0.22 microM glutamate racemase	704192	
5.1.1.3	226.4	-	L-glutamate	pH 7.6, 37°C	746766	
5.1.1.3	780	-	D-glutamate	recombinant wild type enzyme, reactions are conducted at 25°C in the assay mixture that contains 5 mM NAD+, 2.5 mM ADP, 50 mM CHES buffer (pH 9.2), 0.65 mM iodonitrotetrazolium chloride, 37.5 units/ml L-glutamate dehydrogenase, 2 units/ml diaphorase, and 0.05 mM D-glutamate	702331	
5.1.1.3	2160	-	D-glutamate	mutant Q86A, reactions are conducted at 25°C in the assay mixture that contains 5 mM NAD+, 2.5 mM ADP, 50 mM CHES buffer (pH 9.2), 0.65 mM iodonitrotetrazolium chloride, 37.5 units/ml L-glutamate dehydrogenase, 2 units/ml diaphorase, and 0.05 mM D-glutamate	702331	
5.1.1.3	2760	-	D-glutamate	mutant Y221A, reactions are conducted at 25°C in the assay mixture that contains 5 mM NAD+, 2.5 mM ADP, 50 mM CHES buffer (pH 9.2), 0.65 mM iodonitrotetrazolium chloride, 37.5 units/ml L-glutamate dehydrogenase, 2 units/ml diaphorase, and 0.05 mM D-glutamate	702331	
5.1.1.3	3029	-	L-glutamate	pH 7.5, 37°C	728742	
5.1.1.3	5220	-	D-glutamate	mutant K106A, reactions are conducted at 25°C in the assay mixture that contains 5 mM NAD+, 2.5 mM ADP, 50 mM CHES buffer (pH 9.2), 0.65 mM iodonitrotetrazolium chloride, 37.5 units/ml L-glutamate dehydrogenase, 2 units/ml diaphorase, and 0.05 mM D-glutamate	702331	
5.1.1.3	5980	-	D-glutamate	pH 7.5, 37°C	728742	
5.1.1.3	6660	-	D-glutamate	mutant R214A, reactions are conducted at 25°C in the assay mixture that contains 5 mM NAD+, 2.5 mM ADP, 50 mM CHES buffer (pH 9.2), 0.65 mM iodonitrotetrazolium chloride, 37.5 units/ml L-glutamate dehydrogenase, 2 units/ml diaphorase, and 0.05 mM D-glutamate	702331	
5.1.1.3	7440	-	D-glutamate	mutant K29A, reactions are conducted at 25°C in the assay mixture that contains 5 mM NAD+, 2.5 mM ADP, 50 mM CHES buffer (pH 9.2), 0.65 mM iodonitrotetrazolium chloride, 37.5 units/ml L-glutamate dehydrogenase, 2 units/ml diaphorase, and 0.05 mM D-glutamate	702331	
5.1.1.3	7560	-	D-glutamate	mutant R25A, reactions are conducted at 25°C in the assay mixture that contains 5 mM NAD+, 2.5 mM ADP, 50 mM CHES buffer (pH 9.2), 0.65 mM iodonitrotetrazolium chloride, 37.5 units/ml L-glutamate dehydrogenase, 2 units/ml diaphorase, and 0.05 mM D-glutamate	702331	
5.1.1.3	7680	-	D-glutamate	mutant P99A, reactions are conducted at 25°C in the assay mixture that contains 5 mM NAD+, 2.5 mM ADP, 50 mM CHES buffer (pH 9.2), 0.65 mM iodonitrotetrazolium chloride, 37.5 units/ml L-glutamate dehydrogenase, 2 units/ml diaphorase, and 0.05 mM D-glutamate	702331	
5.1.1.3	7980	-	D-glutamate	mutant R214A/K106A, reactions are conducted at 25°C in the assay mixture that contains 5 mM NAD+, 2.5 mM ADP, 50 mM CHES buffer (pH 9.2), 0.65 mM iodonitrotetrazolium chloride, 37.5 units/ml L-glutamate dehydrogenase, 2 units/ml diaphorase, and 0.05 mM D-glutamate	702331	
5.1.1.3	116600	-	L-glutamate	recombinant wild type enzyme, in 10 mM potassium phosphate (pH 8.2) and 0.2 mM dithiothreitol	702331	
5.1.1.3	144000	-	L-glutamate	mutant Q86A, in 10 mM potassium phosphate (pH 8.2) and 0.2 mM dithiothreitol	702331	
5.1.1.3	164400	-	L-glutamate	mutant P99A, in 10 mM potassium phosphate (pH 8.2) and 0.2 mM dithiothreitol	702331	
5.1.1.3	219600	-	L-glutamate	mutant K106A, in 10 mM potassium phosphate (pH 8.2) and 0.2 mM dithiothreitol	702331	
5.1.1.3	252000	-	L-glutamate	mutant Y221A, in 10 mM potassium phosphate (pH 8.2) and 0.2 mM dithiothreitol	702331	
5.1.1.3	278400	-	L-glutamate	mutant R214A/K106A, in 10 mM potassium phosphate (pH 8.2) and 0.2 mM dithiothreitol	702331	
5.1.1.3	294000	-	L-glutamate	mutant R25A, in 10 mM potassium phosphate (pH 8.2) and 0.2 mM dithiothreitol	702331	
5.1.1.3	306000	-	L-glutamate	mutant K29A, in 10 mM potassium phosphate (pH 8.2) and 0.2 mM dithiothreitol	702331	
5.1.1.3	355200	-	L-glutamate	mutant R214A, in 10 mM potassium phosphate (pH 8.2) and 0.2 mM dithiothreitol	702331