4.3.2.10	additional information	-	5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide	pH 7.0, 25°C, kinetic constants are determined for wild-type and mutant enzymes	747285	
4.3.2.10	additional information	-	L-glutamine	pH 7.0, 25°C, kinetic constants are determined for wild-type and mutant enzymes	747285	
4.3.2.10	0.00165	-	L-glutamine	pH 8.0, 37°C, wild-type enzyme	749186	
4.3.2.10	0.00173	-	L-glutamine	pH 8.0, 37°C, HisF mutant enzyme V12A	749186	
4.3.2.10	0.00311	-	L-glutamine	pH 8.0, 37°C, mutant enzyme D98A	749186	
4.3.2.10	0.00328	-	L-glutamine	pH 8.0, 37°C, mutant enzyme V48A	749186	
4.3.2.10	0.00372	-	L-glutamine	pH 8.0, 30°C	747861	
4.3.2.10	0.00372	-	L-glutamine	pH 8.0, 30°C, basal activity	747861	
4.3.2.10	0.0054	-	L-glutamine	pH 8.0, 37°C, HisF mutant enzyme K19A	749186	
4.3.2.10	0.045	-	5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide	30°C, pH 7.0, cosubstrate: L-glutamine, mutant enzyme K258A	747058	
4.3.2.10	0.0571	-	L-glutamine	pH 8.0, 40°C	747861	
4.3.2.10	0.0571	-	L-glutamine	pH 8.0, 40°C, basal activity	747861	
4.3.2.10	0.126	-	5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide	30°C, pH 7.0, cosubstrate: L-glutamine, mutant enzyme K258R	747058	
4.3.2.10	0.139	-	L-glutamine	pH 8.0, 50°C	747861	
4.3.2.10	0.139	-	L-glutamine	pH 8.0, 50°C, basal activity	747861	
4.3.2.10	0.15	-	5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide	30°C, pH 7.0, cosubstrate: NH4+, mutant enzyme R239A	747058	
4.3.2.10	0.18	-	L-glutamine	pH 8.0, 37°C, HisF mutant enzyme D98A, activated by 1 mM 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide	749186	
4.3.2.10	0.204	-	L-glutamine	pH 8.0, 70°C	747861	
4.3.2.10	0.204	-	L-glutamine	pH 8.0, 70°C, basal activity	747861	
4.3.2.10	0.21	-	5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide	30°C, pH 7.0, cosubstrate: NH4+, mutant enzyme R239H	747058	
4.3.2.10	0.212	-	L-glutamine	pH 8.0, 60°C	747861	
4.3.2.10	0.212	-	L-glutamine	pH 8.0, 60°C, basal activity	747861	
4.3.2.10	0.24	-	5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide	30°C, pH 7.0, cosubstrate: L-glutamine, mutant enzyme K360A	747058	
4.3.2.10	0.24	-	L-glutamine	pH 8.0, 37°C, HisF mutant enzyme V48A, activated by 1 mM 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide	749186	
4.3.2.10	0.29	-	5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide	30°C, pH 7.0, cosubstrate: L-glutamine, mutant enzyme K360R	747058	
4.3.2.10	0.3	-	5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide	30°C, pH 7.0, cosubstrate: NH4+, mutant enzyme R239K	747058	
4.3.2.10	0.31	-	5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide	30°C, pH 7.0, cosubstrate: NH4+, mutant enzyme K360R	747058	
4.3.2.10	0.4	-	L-glutamine	pH 8.0, 25°C, wild-type HisH-HisF complex	748148	
4.3.2.10	0.57	-	L-glutamine	pH 8.0, 37°C, HisF mutant enzyme K19A, activated by 1 mM 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide	749186	
4.3.2.10	0.6	-	NH4+	pH 7.0, 30°C	749228	
4.3.2.10	0.7	-	5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide	30°C, pH 7.0, cosubstrate: NH4+, mutant enzyme K360A	747058	
4.3.2.10	0.8	-	5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide	pH 8.0, 25°C, wild-type HisH-HisF complex	748148	
4.3.2.10	0.845	-	5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide	30°C, pH 7.0, cosubstrate: NH4+, wild-type enzyme	747058	
4.3.2.10	1.88	-	L-glutamine	pH 8.0, 37°C, HisF mutant enzyme V12A, activated by 1 mM 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide	749186	
4.3.2.10	2	-	NH4+	pH 8.5, 25°C, ammonia-dependent reaction of isolated HisF subunit, wild-type	748148	
4.3.2.10	2.2	-	5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide	pH 8.5, 25°C, ammonia-dependent reaction of isolated HisF subunit, wild-type	748148	
4.3.2.10	3.62	-	L-glutamine	pH 8.0, 37°C, wild-type enzyme, activated by 1 mM 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide	749186	
4.3.2.10	3.9	-	5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide	30°C, pH 7.0, cosubstrate: L-glutamine, mutant enzyme R239H	747058	
4.3.2.10	3.9	-	5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide	pH 7.0, 30°C	749228	
4.3.2.10	4.09	-	L-glutamine	pH 8.0, 30°C, activated by 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide	747861	
4.3.2.10	4.3	-	5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide	30°C, pH 7.0, cosubstrate: L-glutamine, mutant enzyme R239A	747058	
4.3.2.10	4.37	-	L-glutamine	pH 8.0, 40°C, activated by 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide	747861	
4.3.2.10	4.89	-	L-glutamine	pH 8.0, 50°C, activated by 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide	747861	
4.3.2.10	5	-	5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide	30°C, pH 7.0, cosubstrate: NH4+, mutant enzyme K258R	747058	
4.3.2.10	5.2	-	L-glutamine	pH 7.0, 30°C	749228	
4.3.2.10	5.4	-	5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide	30°C, pH 7.0, cosubstrate: L-glutamine, wild-type enzyme	747058	
4.3.2.10	5.4	-	5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide	30°C, pH 7.0, cosubstrate: NH4+, mutant enzyme K258A	747058	
4.3.2.10	5.5	-	L-glutamine	pH 8.0, 60°C, activated by 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide	747861	
4.3.2.10	5.92	-	L-glutamine	pH 8.0, 70°C, activated by 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide	747861	
4.3.2.10	6.1	-	5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide	pH 8.0, 30°C, cosubstrate: NH4+	747052	
4.3.2.10	8.5	-	5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide	pH 8.0, 30°C, cosubstrate: L-glutamine	747052	
4.3.2.10	8.7	-	5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide	30°C, pH 7.0, cosubstrate: L-glutamine, mutant enzyme R239K	747058	
4.3.2.10	8.8	-	NH4+	pH 8.0, 30°C	747052	
4.3.2.10	9.1	-	L-glutamine	pH 8.0, 30°C	747052