4.1.3.3 0.0002 - N-acetyl-L-neuraminic acid pH 7.5, 25°C, wild-type enzyme 666750 4.1.3.3 0.0045 - N-acetyl-L-neuraminic acid pH 7.5, 25°C, mutant enzyme Y98H/F115L/V251I 666750 4.1.3.3 0.0068 - N-acetyl-L-neuraminic acid pH 7.5, 25°C, mutant enzyme Y98H/F115L/V251I/V265I 666750 4.1.3.3 0.013 - N-acetylneuraminate mutant Y137F enzyme, pH 7.4, 30°C 726551 4.1.3.3 0.013 - N-acetylneuraminate purified recombinant mutant K165C, pH 7.4, 30°C 727317 4.1.3.3 0.015 - N-acetylneuraminate purified recombinant mutant K165-gamma-thialysine, pH 6.8, 30°C 727317 4.1.3.3 0.02 - N-acetyl-L-neuraminic acid pH 7.5, 25°C, mutant enzyme E60A/Y98H/F115L/N153Y/D150G/V251I/V265I/Y281C/N153Y 666750 4.1.3.3 0.02 - N-acetyl-L-neuraminic acid pH 7.5, 25°C, mutant enzyme Y98H/F115L/N153Y/V251I/V265I/Y281C 666750 4.1.3.3 0.023 - N-acetylneuraminate purified recombinant mutant K165-gamma-thialysine, pH 7.4, 30°C 727317 4.1.3.3 0.032 - N-acetylneuraminate mutant enzyme G211S, at pH 7.0 and 37°C 749150 4.1.3.3 0.036 - N-acetylneuraminate purified recombinant wild-type enzyme, pH 7.4, 30°C 727317 4.1.3.3 0.04 - N-acetylneuraminate purified recombinant wild-type enzyme, pH 6.8, 30°C 727317 4.1.3.3 0.057 0.65 (2R,3R,4R)-6-[(aminooxy)carbonyl]-3,4,6-trihydroxy-N-propyltetrahydro-2H-pyran-2-carboxamide mutant enzyme E192N 663630 4.1.3.3 0.1 - (5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid E192D mutant protein, pH 7.5, 25°C 715921 4.1.3.3 0.13 - N-acetylneuraminate mutant enzyme P193Y, at pH 7.0 and 37°C 749150 4.1.3.3 0.29 - N-acetylneuraminate mutant enzyme L199N/I229D, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C 702067 4.1.3.3 0.42 - N-acetylneuraminic acid E192P mutant protein, pH 7.5, 25°C 715921 4.1.3.3 0.43 - (5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid E192S mutant protein, pH 7.5, 25°C 715921 4.1.3.3 0.44 - (2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid pH 7.5, 25°C, mutant enzyme S208G 666805 4.1.3.3 0.47 - N-acetylneuraminate mutant enzyme G210S/Y213G, at pH 7.0 and 37°C 749150 4.1.3.3 0.63 - (5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid E192H mutant protein, pH 7.5, 25°C 715921 4.1.3.3 0.664 - N-acetylneuraminate mutant enzyme L199N/I229N, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C 702067 4.1.3.3 0.82 - (5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid E192P mutant protein, pH 7.5, 25°C 715921 4.1.3.3 0.83 - N-acetylneuraminic acid E192S mutant protein, pH 7.5, 25°C 715921 4.1.3.3 0.9 - N-acetylneuraminate wild type enzyme, at pH 7.0 and 37°C 749150 4.1.3.3 0.91 - (2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid pH 7.5, 25°C, mutant enzyme D191P 666805 4.1.3.3 0.99 - pyruvate mutant enzyme P193Y, at pH 7.0 and 37°C 749150 4.1.3.3 1.1 - L-3-deoxy-manno-2-octulosonic acid pH 7.5, 25°C, mutant enzyme Y98H/F115L 666750 4.1.3.3 1.1 - L-3-deoxy-manno-2-octulosonic acid pH 7.5, 25°C, wild-type enzyme 666750 4.1.3.3 1.17 - (5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid E192M mutant protein, pH 7.5, 25°C 715921 4.1.3.3 1.17 - N-acetylneuraminic acid E192M mutant protein, pH 7.5, 25°C 715921 4.1.3.3 1.2 - D-3-deoxy-manno-2-octulosonic acid pH 7.5, 25°C, wild-type enzyme 666750 4.1.3.3 1.22 - (2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid pH 7.5, 25°C, wild-type enzyme 666805 4.1.3.3 1.22 - (5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid wild type (E192), pH 7.5, 25°C 715921 4.1.3.3 1.23 - (2R,3R,4R)-6-[(aminooxy)carbonyl]-3,4,6-trihydroxy-N-propyltetrahydro-2H-pyran-2-carboxamide wild-type enzyme 663630 4.1.3.3 1.47 - (5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid E192F mutant protein, pH 7.5, 25°C 715921 4.1.3.3 1.5 - D-3-deoxy-manno-2-octulosonic acid pH 7.5, 25°C, mutant enzyme Y98H/F115L 666750 4.1.3.3 1.5 - N-acetyl-D-neuraminic acid pH 7.5, 25°C, mutant enzyme E60A/Y98H/F115L/N153Y/D150G/V251I/V265I/Y281C/N153Y 666750 4.1.3.3 1.58 - (2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid pH 7.5, 25°C, mutant enzyme E192V 666805 4.1.3.3 1.58 - (5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid E192V mutant protein, pH 7.5, 25°C 715921 4.1.3.3 1.7 - N-acetyl-D-neuraminic acid pH 7.5, 25°C, mutant enzyme Y98H/F115L/N153Y/V251I/V265I/Y281C 666750 4.1.3.3 1.8 - pyruvate - 690576 4.1.3.3 1.85 - (2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid pH 7.5, 25°C, mutant enzyme E192S 666805 4.1.3.3 1.9 - L-3-deoxy-manno-2-octulosonic acid pH 7.5, 25°C, mutant enzyme Y98H/F115L/V251I 666750 4.1.3.3 1.9 - N-acetylneuraminate wild type enzyme, at pH 7.0 and 37°C 749150 4.1.3.3 1.9 - pyruvate - 690576 4.1.3.3 1.97 - N-acetyl-D-neuraminic acid pH 7.5, 25°C, mutant enzyme E192V 666805 4.1.3.3 2 - N-acetylneuraminic acid E192D mutant protein, pH 7.5, 25°C 715921 4.1.3.3 2 - N-acetylneuraminic acid E192H mutant protein, pH 7.5, 25°C 715921 4.1.3.3 2 - N-acetylneuraminic acid E192V mutant protein, pH 7.5, 25°C 715921 4.1.3.3 2.17 - (5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid E192N mutant protein, pH 7.7.5, 25°C 715921 4.1.3.3 2.18 - (2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid pH 7.5, 25°C, mutant enzyme E192N 666805 4.1.3.3 2.2 - N-acetyl-D-neuraminic acid pH 7.5, 25°C, mutant enzyme Y98H/F115L/V251I/V265I 666750 4.1.3.3 2.4 - N-acetyl-D-mannosamine mutant enzyme P193Y, at pH 7.0 and 37°C 749150 4.1.3.3 2.5 - L-3-deoxy-manno-2-octulosonic acid pH 7.5, 25°C, mutant enzyme Y98H/F115L/V251I/V265I 666750 4.1.3.3 2.67 - N-acetylneuraminic acid E192F mutant protein, pH 7.5, 25°C 715921 4.1.3.3 2.77 - N-acetyl-D-neuraminic acid pH 7.5, 25°C, mutant enzyme E192N 666805 4.1.3.3 2.8 - D-3-deoxy-manno-2-octulosonic acid pH 7.5, 25°C, mutant enzyme Y98H/F115L/V251I 666750 4.1.3.3 2.83 - (5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid E192Q mutant protein, pH 7.5, 25°C 715921 4.1.3.3 2.83 - N-acetylneuraminic acid E192N mutant protein, pH 7.7.5, 25°C 715921 4.1.3.3 2.833 - sialic acid mutant enzyme E192N 663630 4.1.3.3 2.9 - pyruvate recombinant His-taggged enzyme, pH 7.0, 37°C 727656 4.1.3.3 3 - pyruvate mutant enzyme G210S/Y213G, at pH 7.0 and 37°C 749150 4.1.3.3 3 6 sialic acid mutant enzyme E192N 663630 4.1.3.3 3.15 - N-acetylneuraminate mutant enzyme L171N/I229N, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C 702067 4.1.3.3 3.2 - D-3-deoxy-manno-2-octulosonic acid pH 7.5, 25°C, mutant enzyme Y98H/F115L/V251I/V265I 666750 4.1.3.3 3.9 - pyruvate wild type enzyme, at pH 7.0 and 37°C 749150 4.1.3.3 4 - N-acetylneuraminate recombinant His-taggged enzyme, pH 7.0, 37°C 727656 4.1.3.3 4.15 - N-acetyl-D-neuraminic acid pH 7.5, 25°C, mutant enzyme S208G 666805 4.1.3.3 4.17 - N-acetylneuraminate wild-type enzyme, pH 7.4, 30°C 726551 4.1.3.3 4.2 - L-3-deoxy-manno-2-octulosonic acid pH 7.5, 25°C, mutant enzyme Y98H/F115L/N153Y/V251I/V265I/Y281C 666750 4.1.3.3 4.25 - N-acetyl-D-neuraminic acid pH 7.5, 25°C, mutant enzyme E192S 666805 4.1.3.3 4.3 - N-acetyl-D-neuraminic acid pH 7.5, 25°C, wild-type enzyme 666805 4.1.3.3 4.33 - N-acetylneuraminic acid wild type (E192), pH 7.5, 25°C 715921 4.1.3.3 4.33 - sialic acid wild-type enzyme 663630 4.1.3.3 4.5 - N-acetyl-D-mannosamine recombinant His-taggged enzyme, pH 7.0, 37°C 727656 4.1.3.3 4.6 - N-acetyl-D-mannosamine wild type enzyme, at pH 7.0 and 37°C 749150 4.1.3.3 4.69 - N-acetylneuraminate mutant enzyme I229D, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C 702067 4.1.3.3 5 - N-acetylneuraminic acid E192Q mutant protein, pH 7.5, 25°C 715921 4.1.3.3 5.1 - D-3-deoxy-manno-2-octulosonic acid pH 7.5, 25°C, mutant enzyme Y98H/F115L/N153Y/V251I/V265I/Y281C 666750 4.1.3.3 5.4 - N-acetyl-D-mannosamine wild type enzyme, at pH 7.0 and 37°C 749150 4.1.3.3 5.49 - N-acetylneuraminate mutant enzyme L171R, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C 702067 4.1.3.3 5.6 - pyruvate wild type enzyme, at pH 7.0 and 37°C 749150 4.1.3.3 5.67 - N-acetyl-D-mannosamine at pH 7.5 and 37°C 749312 4.1.3.3 6.1 - pyruvate at pH 7.5 and 37°C 749312 4.1.3.3 6.75 - N-acetylneuraminate wild type enzyme, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C 702067 4.1.3.3 6.8 - N-acetylneuraminic acid pH 7.2, 25°C, L142R/Y190D/E192A mutant 653594 4.1.3.3 7.4 - N-acetylneuraminate mutant enzyme I229N, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C 702067 4.1.3.3 7.7 - N-acetylneuraminic acid pH 7.2, 25°C, wild-type 653594 4.1.3.3 7.72 - N-acetylneuraminate mutant enzyme I229R, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C 702067 4.1.3.3 7.94 - N-acetylneuraminate mutant enzyme L199N, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C 702067 4.1.3.3 8.9 - D-3-deoxy-manno-2-octulosonic acid pH 7.5, 25°C, mutant enzyme E60A/Y98H/F115L/N153Y/D150G/V251I/V265I/Y281C/N153Y 666750 4.1.3.3 8.9 - N-acetylneuraminate mutant enzyme L199N/I229R, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C 702067 4.1.3.3 9 - N-acetyl-D-mannosamine - 690576 4.1.3.3 9.3 - N-acetylneuraminate at pH 7.5 and 37°C 749312 4.1.3.3 9.7 - N-acetyl-D-neuraminic acid pH 7.5, 25°C, mutant enzyme Y98H/F115L/V251I 666750 4.1.3.3 10 - N-acetylneuraminate - 690576 4.1.3.3 10.3 - N-acetyl-D-neuraminic acid pH 7.5, 25°C, Y98H/F115L 666750 4.1.3.3 10.4 - L-3-deoxy-manno-2-octulosonic acid pH 7.5, 25°C, mutant enzyme E60A/Y98H/F115L/N153Y/D150G/V251I/V265I/Y281C/N153Y 666750 4.1.3.3 10.5 - N-acetyl-D-neuraminic acid pH 7.5, 25°C, wild-type enzyme 666750 4.1.3.3 10.83 - (2R,3R,4R)-6-[(aminooxy)carbonyl]-3,4,6-trihydroxy-N-propyltetrahydro-2H-pyran-2-carboxamide mutant enzyme E192N 663630 4.1.3.3 11 - N-acetyl-D-mannosamine - 690576 4.1.3.3 11.87 - N-acetylneuraminate mutant enzyme L199R/I229N, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C 702067 4.1.3.3 12.1 - N-acetylneuraminic acid pH 7.2, 25°C, L142R mutant 653594 4.1.3.3 15.3 - N-acetyl-D-mannosamine mutant enzyme G210S/Y213G, at pH 7.0 and 37°C 749150 4.1.3.3 16 - N-acetylneuraminate - 690576 4.1.3.3 16.7 - N-acetylneuraminic acid - 33315 4.1.3.3 22.1 - N-acetylneuraminate at 30°C, pH not specified in the publication 747776 4.1.3.3 40.67 - N-acetyl-D-mannosamine at pH 8.5 and 37°C 749312 4.1.3.3 42.58 - pyruvate at pH 8.5 and 37°C 749312 4.1.3.3 44.2 - N-acetylneuraminate at pH 8.5 and 37°C 749312