3.4.24.23 additional information - additional information kcat-value decreases from 337 mg/ml x M x s to 305 mg/ml x M x s upon nitration of 4-5 Tyr residues of enzyme, pH 7.5, 25°C 667492 3.4.24.23 additional information - additional information turnover of highly active and selective substrates produced by using bacteriophage peptide display libraries 31078 3.4.24.23 0.00051 - dansyl-PLALWAR pH 7.5, 25°C, mutant E198A 649827 3.4.24.23 0.0036 - dansyl-PLALWAR pH 7.5, 25°C, mutant E198Q 649827 3.4.24.23 0.014 - dansyl-PLALWAR pH 7.5, 25°C, mutant E198C 649827 3.4.24.23 0.222 - Gly-Pro-Gln-Gly-Ile-Ala-Gly-Gln - 31070 3.4.24.23 0.472 - Gly-Pro-Gln-Ala-Ile-Ala-Gly-Gln - 31070 3.4.24.23 0.61 - dansyl-PLALWAR pH 7.5, 25°C, mutant E198D 649827 3.4.24.23 1.17 - Gly-Pro-Gln-Gly-Ile-Ala-Met-Gln - 31070 3.4.24.23 1.61 - (7-methoxycoumarin-4-yl)acetyl-LPro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2 pH 7.5, 25°C, mutant Y219C 717745 3.4.24.23 1.92 - Gly-Pro-Met-Gly-Ile-Ala-Gly-Gln - 31070 3.4.24.23 2.47 - Gly-Pro-Gln-Gly-Leu-Ala-Gly-Gln - 31070 3.4.24.23 2.7 - dansyl-PLALWAR pH 7.5, 25°C, wild-type 649827 3.4.24.23 3.5 - 2,4-dinitrophenyl-Pro-Leu-Gly-Leu-Trp-Ala-D-Arg - 31080 3.4.24.23 3.53 - (7-methoxycoumarin-4-yl)acetyl-LPro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2 pH 7.5, 25°C, mutant Y219A 717745 3.4.24.23 5 - 2,4-Dinitrophenyl-Arg-Pro-Leu-Ala-Leu-Trp-Arg-Ser - 31080 3.4.24.23 5.41 - (7-methoxycoumarin-4-yl)acetyl-LPro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2 pH 7.5, 25°C, mutant Y219S 717745 3.4.24.23 6.08 - dansyl-PLALWAR pH 7.5, 25°C, mutant E198D 649827 3.4.24.23 6.18 - (7-methoxycoumarin-4-yl)acetyl-LPro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2 pH 7.5, 25°C, mutant Y219F 717745 3.4.24.23 6.35 - (7-methoxycoumarin-4-yl)acetyl-LPro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2 pH 7.5, 25°C, mutant Y219D 717745 3.4.24.23 6.43 - (7-methoxycoumarin-4-yl)acetyl-LPro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2 pH 7.5, 25°C, wild-type enzyme 717745