3.4.21.27 0.00065 - factor IX 37°C, recombinant peptide FXI/E361-V607 683167 3.4.21.27 0.083 - factor IX 10-mer at pH 7.4 and 37°C 753069 3.4.21.27 0.2 - factor IX in 50 mM Tris-HCl, 150 mM NaCl, 5 mM CaCl2, 0.5% (w/v) bovine serum albumin, pH 7.3, at 37°C 717844 3.4.21.27 0.2 - factor IX kcat less than 0.2 s-1, in 50 mM Tris-HCl, 150 mM NaCl, 5 mM CaCl2, 0.5% (w/v) bovine serum albumin, pH 7.3, at 37°C 717844 3.4.21.27 0.2 - factor IX kcat less than 0.2 s-1, mutant enzyme E98A, in 50 mM Tris-HCl, 150 mM NaCl, 5 mM CaCl2, 0.5% (w/v) bovine serum albumin, pH 7.3, at 37°C 717844 3.4.21.27 0.2 - factor IX kcat less than 0.2 s-1, mutant enzyme E98D, in 50 mM Tris-HCl, 150 mM NaCl, 5 mM CaCl2, 0.5% (w/v) bovine serum albumin, pH 7.3, at 37°C 717844 3.4.21.27 0.2 - factor IX kcat less than 0.2 s-1, mutant enzyme E98V, in 50 mM Tris-HCl, 150 mM NaCl, 5 mM CaCl2, 0.5% (w/v) bovine serum albumin, pH 7.3, at 37°C 717844 3.4.21.27 0.2 - factor IX kcat less than 0.2 s-1, mutant enzyme Y143A, in 50 mM Tris-HCl, 150 mM NaCl, 5 mM CaCl2, 0.5% (w/v) bovine serum albumin, pH 7.3, at 37°C 717844 3.4.21.27 0.2 - factor IX mutant enzyme I151A, in 50 mM Tris-HCl, 150 mM NaCl, 5 mM CaCl2, 0.5% (w/v) bovine serum albumin, pH 7.3, at 37°C 717844 3.4.21.27 0.268 - pyroGlu-Pro-Arg-4-nitroanilide pH 7.4, 37°C, recombinant wild-type enzyme 683682 3.4.21.27 0.334 - factor IX 37°C, recombinant wild-type enzyme 683167 3.4.21.27 0.49 - factor fIX fXIa 669347 3.4.21.27 0.55 - factor IX pH 7.4, 37°C, recombinant wild-type enzyme 683682 3.4.21.27 0.66 - factor IX at 22°C 667583 3.4.21.27 0.73 - factor IX wild type enzyme, in 50 mM Tris-HCl, 150 mM NaCl, 5 mM CaCl2, 0.5% (w/v) bovine serum albumin, pH 7.3, at 37°C 717844 3.4.21.27 0.91 - prochemerin chem163S at pH 7.4 and 37°C 753069 3.4.21.27 3.4 - factor IX FXIa/PKA4, 37°C 650724 3.4.21.27 5.7 - factor IX FXIa/PKA4-Gly326, 37°C 650724 3.4.21.27 5.8 - factor IX factor FXIa, 37°C 650724 3.4.21.27 5.8 - L-pyroGlu-L-Pro-L-Arg-4-nitroanilide mutant enzyme E98D, in 50 mM Tris-HCl, 150 mM NaCl, 5 mM CaCl2, 0.5% (w/v) bovine serum albumin, pH 7.3, at 37°C 717844 3.4.21.27 7 - L-pyroGlu-L-Pro-L-Arg-4-nitroanilide mutant enzyme Y143A, in 50 mM Tris-HCl, 150 mM NaCl, 5 mM CaCl2, 0.5% (w/v) bovine serum albumin, pH 7.3, at 37°C 717844 3.4.21.27 8 - Factor XI pH 7.3, 37°C 652013 3.4.21.27 8 - L-pyroGlu-L-Pro-L-Arg-4-nitroanilide mutant enzyme R3704A, in 50 mM Tris-HCl, 150 mM NaCl, 5 mM CaCl2, 0.5% (w/v) bovine serum albumin, pH 7.3, at 37°C 717844 3.4.21.27 14 - L-pyroGlu-L-Pro-L-Arg-4-nitroanilide mutant enzyme K192R, in 50 mM Tris-HCl, 150 mM NaCl, 5 mM CaCl2, 0.5% (w/v) bovine serum albumin, pH 7.3, at 37°C 717844 3.4.21.27 21 - L-pyroGlu-L-Pro-L-Arg-4-nitroanilide mutant enzyme E98V, in 50 mM Tris-HCl, 150 mM NaCl, 5 mM CaCl2, 0.5% (w/v) bovine serum albumin, pH 7.3, at 37°C 717844 3.4.21.27 29 - L-pyroGlu-L-Pro-L-Arg-4-nitroanilide mutant enzyme Y5901A, in 50 mM Tris-HCl, 150 mM NaCl, 5 mM CaCl2, 0.5% (w/v) bovine serum albumin, pH 7.3, at 37°C 717844 3.4.21.27 30 - L-pyroGlu-L-Pro-L-Arg-4-nitroanilide mutant enzyme K192Q, in 50 mM Tris-HCl, 150 mM NaCl, 5 mM CaCl2, 0.5% (w/v) bovine serum albumin, pH 7.3, at 37°C 717844 3.4.21.27 42 - L-pyroGlu-L-Pro-L-Arg-4-nitroanilide mutant enzyme Y5901V, in 50 mM Tris-HCl, 150 mM NaCl, 5 mM CaCl2, 0.5% (w/v) bovine serum albumin, pH 7.3, at 37°C 717844 3.4.21.27 58 - L-pyroGlu-L-Pro-L-Arg-4-nitroanilide mutant enzyme I151A, in 50 mM Tris-HCl, 150 mM NaCl, 5 mM CaCl2, 0.5% (w/v) bovine serum albumin, pH 7.3, at 37°C 717844 3.4.21.27 67 - L-pyroglutamyl-L-prolyl-L-arginyl-p-nitroanilide fXIaCD 669347 3.4.21.27 86 - L-pyroGlu-L-Pro-L-Arg-4-nitroanilide mutant enzyme K192E, in 50 mM Tris-HCl, 150 mM NaCl, 5 mM CaCl2, 0.5% (w/v) bovine serum albumin, pH 7.3, at 37°C 717844 3.4.21.27 95 - L-pyroGlu-L-Pro-L-Arg-4-nitroanilide mutant enzyme E98A, in 50 mM Tris-HCl, 150 mM NaCl, 5 mM CaCl2, 0.5% (w/v) bovine serum albumin, pH 7.3, at 37°C 717844 3.4.21.27 95 - L-pyroGlu-L-Pro-L-Arg-4-nitroanilide mutant enzyme K192A, in 50 mM Tris-HCl, 150 mM NaCl, 5 mM CaCl2, 0.5% (w/v) bovine serum albumin, pH 7.3, at 37°C 717844 3.4.21.27 98 - L-pyroglutamyl-L-prolyl-L-argininyl-p-nitroanilide fXIa-catalytic domain-K170A mutant 691011 3.4.21.27 110.2 - D-Ile-Pro-Arg-4-nitroanilide recombinant wild-type enzyme, pH 7.5, 37°C 683169 3.4.21.27 113 - S-2366 37°C, recombinant wild-type enzyme 683167 3.4.21.27 115 - L-pyroglutamyl-L-prolyl-L-argininyl-p-nitroanilide fXIa-catalytic domain-K170A/R171A/R173A mutant 691011 3.4.21.27 115 - L-pyroglutamyl-L-prolyl-L-argininyl-p-nitroanilide fXIa-catalytic domain-R171A mutant 691011 3.4.21.27 116 - L-pyroglutamyl-L-prolyl-L-argininyl-p-nitroanilide fXIa-catalytic domain-K179A mutant 691011 3.4.21.27 117 - L-pyroglutamyl-L-prolyl-L-argininyl-p-nitroanilide fXIa-catalytic domain-K175A mutant 691011 3.4.21.27 117 - L-pyroglutamyl-L-prolyl-L-argininyl-p-nitroanilide fXIa-catalytic domain-R173A mutant 691011 3.4.21.27 117 - L-pyroglutamyl-L-prolyl-L-arginyl-p-nitroanilide fXIa 669347 3.4.21.27 119 - L-pyroglutamyl-L-prolyl-L-argininyl-p-nitroanilide fXIa-catalytic domain-wild type 691011 3.4.21.27 121 - S-2366 37°C, recombinant peptide FXI/E361-V607 683167 3.4.21.27 145 - pyroGlu-Pro-Arg-4-nitroanilide recombinant wild-type enzyme, pH 7.5, 37°C 683169 3.4.21.27 148 - L-pyroglutamyl-L-prolyl-L-argininyl-p-nitroanilide fXIa-R37Q mutant 691011 3.4.21.27 148 - L-pyroglutamyl-L-prolyl-L-argininyl-p-nitroanilide fXIa-wild type 691011 3.4.21.27 201 - L-pyroGlu-L-Pro-L-Arg-4-nitroanilide wild type enzyme, in 50 mM Tris-HCl, 150 mM NaCl, 5 mM CaCl2, 0.5% (w/v) bovine serum albumin, pH 7.3, at 37°C 717844 3.4.21.27 250 - L-pyroglutamyl-L-Pro-L-Arg-4-nitroanilide FXIa/PKA4 650724 3.4.21.27 310 - L-pyroglutamyl-L-Pro-L-Arg-4-nitroanilide factor FXIa 650724 3.4.21.27 350 - pyroGlu-Pro-Arg-p-nitroanilide - 36532 3.4.21.27 430 - L-pyroglutamyl-L-Pro-L-Arg-4-nitroanilide FXIa/PKA4-Gly326 650724