2.7.3.3 additional information - additional information - 661152 2.7.3.3 0.00229 - ATP mutant enzyme L65G 663322 2.7.3.3 0.00229 - L-arginine mutant enzyme L65G 663322 2.7.3.3 0.005 - ATP mutant enzyme E314V 663322 2.7.3.3 0.005 - L-arginine mutant enzyme E314V 663322 2.7.3.3 0.0329 - ATP mutant enzyme D62_F63delinsDGF 663322 2.7.3.3 0.0329 - L-arginine mutant enzyme D62_F63delinsDGF 663322 2.7.3.3 0.08 - L-Arg mutant enzyme S312G/E314V/F315D/E317A/S318A/G321S 674037 2.7.3.3 0.093 - Nomega-phospho-L-Arg at pH 8.0 and 25°C 722542 2.7.3.3 0.19 - ATP mutant enzyme H284A, pH and temperature not specified in the publication 761907 2.7.3.3 0.19 - L-arginine mutant enzyme H284A, pH and temperature not specified in the publication 761907 2.7.3.3 0.27 - ADP mutant enzyme E225Q/E314Q 662144 2.7.3.3 0.27 - N5-(N-phosphonocarbamimidoyl)-L-ornithine mutant enzyme E225Q/E314Q 662144 2.7.3.3 0.34 - ADP mutant enzyme E225D 662144 2.7.3.3 0.34 - N5-(N-phosphonocarbamimidoyl)-L-ornithine mutant enzyme E225D 662144 2.7.3.3 0.37 - ADP mutant enzyme E314Q 662144 2.7.3.3 0.37 - N5-(N-phosphonocarbamimidoyl)-L-ornithine mutant enzyme E314D 662144 2.7.3.3 0.443 - ATP mutant enzyme R193G 663322 2.7.3.3 0.443 - L-arginine mutant enzyme R193G 663322 2.7.3.3 0.45 - ADP mutant enzyme E225Q 662144 2.7.3.3 0.45 - N5-(N-phosphonocarbamimidoyl)-L-ornithine mutant enzyme E225Q 662144 2.7.3.3 0.833 - L-Arg mutant enzyme F315Y 674037 2.7.3.3 0.913 - ATP mutant enzyme D62G 663322 2.7.3.3 0.913 - L-arginine mutant enzyme D62G 663322 2.7.3.3 1.02 - L-Arg mutant enzyme F315A 674037 2.7.3.3 1.1 - L-Arg wild type enzyme 674037 2.7.3.3 1.11 - ADP pH 8, 25°C 686222 2.7.3.3 1.11 - omega-N-phospho-L-arginine pH 8, 25°C 686222 2.7.3.3 2 3.7 D-Arg the reaction mixture contains 100 mM Tris/HCl (pH 8), 750 mM KCl, 250 mM Mg-acetate, 25 mM phosphoenolpyruvate made up in 100 mM imidazole/HCl (pH 7), 5 mM NADH made up in Tris/HCl (pH 8), pyruvate kinase/lactate dehydrogenase mixture made up in 100 mM imidazole/HCl (pH 7), 100 mM ATP made up in 100 mM imidazole/HCl (pH 7), and recombinant enzyme, at 25°C 676995 2.7.3.3 2.02 - ATP pH 8, 25°C 674014 2.7.3.3 2.02 - L-Arg in 100 mM Tris/HCl (pH 8), 750 mM KCl, 250 mM Mg-acetate, 25 mM phosphoenolpyruvate, at 25°C 674014 2.7.3.3 2.02 - L-Arg pH 8, 25°C 674014 2.7.3.3 2.17 - ADP mutant enzyme E314D 662144 2.7.3.3 2.17 - N5-(N-phosphonocarbamimidoyl)-L-ornithine mutant enzyme E314S 662144 2.7.3.3 2.73 - L-Arg two-domain enzyme, in 100 mM Tris-HCl (pH 8.0), at 10°C 723082 2.7.3.3 3.31 - ATP mutant enzyme F63G 663322 2.7.3.3 3.31 - L-arginine mutant enzyme F63G 663322 2.7.3.3 3.97 - L-Arg mutant enzyme F315H 674037 2.7.3.3 4.3 - L-Arg two-domain enzyme, in 100 mM Tris-HCl (pH 8.0), at 25°C 723082 2.7.3.3 4.38 - L-Arg mutant enzyme Q53A/D57A, in 100 mM Tris, pH 8.0, at 30°C 721428 2.7.3.3 5.54 - L-Arg mutant enzyme S312R/F315H/V319E 674037 2.7.3.3 5.8 - L-Arg 25°C 676995 2.7.3.3 6.74 - L-arginine pH 7.5, 30°C 760909 2.7.3.3 7.4 - L-Arg isolated domain 2, in 100 mM Tris-HCl (pH 8.0), at 10°C 723082 2.7.3.3 8.8 - L-Arg - 673166 2.7.3.3 8.92 - ATP 25°C, H60G mutant of domain 2 642468 2.7.3.3 8.92 - L-Arg 25°C, H60G mutant of domain 2 642468 2.7.3.3 9.22 - ATP mutant enzyme Y89R 663322 2.7.3.3 9.22 - L-arginine mutant enzyme Y89R 663322 2.7.3.3 9.53 - ATP 25°C, D197G mutant of domain 2 642468 2.7.3.3 9.53 - L-Arg 25°C, D197G mutant of domain 2 642468 2.7.3.3 10.26 - L-Arg mutant enzyme D57A, in 100 mM Tris, pH 8.0, at 30°C 721428 2.7.3.3 11.4 - ATP pH 8.0, 25°C, recombinant enzyme 738080 2.7.3.3 11.4 - L-arginine pH 8.0, 25°C, recombinant enzyme 738080 2.7.3.3 12.7 - L-arginine pH 7.5, 30°C, arginine kinase TcAK2 760906 2.7.3.3 13.09 - L-Arg mutant enzyme Q53A, in 100 mM Tris, pH 8.0, at 30°C 721428 2.7.3.3 13.7 - L-Arg isolated domain 2, in 100 mM Tris-HCl (pH 8.0), at 25°C 723082 2.7.3.3 15.67 - L-Arg mutant enzyme F315Y 674037 2.7.3.3 15.95 - L-Arg mutant enzyme D57E, in 100 mM Tris, pH 8.0, at 30°C 721428 2.7.3.3 18.09 - ATP mutant enzyme H64G 663322 2.7.3.3 18.09 - L-arginine mutant enzyme H64G 663322 2.7.3.3 18.1 - ATP 25°C, H60R mutant of domain 2 642468 2.7.3.3 18.1 - L-Arg 25°C, H60R mutant of domain 2 642468 2.7.3.3 18.6 - ATP wild-type enzyme, pH and temperature not specified in the publication 761907 2.7.3.3 18.6 - L-arginine wild-type enzyme, pH and temperature not specified in the publication 761907 2.7.3.3 18.66 - L-Arg wild type enzyme 674037 2.7.3.3 22 - Nomega-phospho-L-arginine pH 7.5, 30°C, arginine kinase TcAK1 760906 2.7.3.3 22.8 - ATP mutant enzyme S282G 663322 2.7.3.3 22.8 - L-arginine mutant enzyme S282G 663322 2.7.3.3 22.9 - ATP wild-type two-domain enzyme 686224 2.7.3.3 23.33 - L-Arg mutant enzyme Q53E, in 100 mM Tris, pH 8.0, at 30°C 721428 2.7.3.3 24.4 - ATP pH 7.9, 25°C, mutant enzyme N62G/K193G 661753 2.7.3.3 24.4 - L-arginine pH 7.9, 25°C, mutant enzyme N62G/K193G 661753 2.7.3.3 25.3 - ATP pH 7.9, 25°C, mutant enzyme N62G 661753 2.7.3.3 25.3 - L-arginine pH 7.9, 25°C, mutant enzyme N62G 661753 2.7.3.3 25.7 - ATP wild-type enzyme 663322 2.7.3.3 25.7 - L-Arg native wild type enzyme, in 100 mM Tris, pH 8.0, at 30°C 721428 2.7.3.3 25.7 - L-arginine wild-type enzyme 663322 2.7.3.3 27.3 - L-(+)-(S)-canavanine - 642451 2.7.3.3 29.18 - ATP - 673493 2.7.3.3 29.18 - L-Arg - 673493 2.7.3.3 29.93 - L-Arg recombinant wild type enzyme, in 100 mM Tris, pH 8.0, at 30°C 721428 2.7.3.3 32.4 - ATP pH 7.9, 25°C, mutant enzyme K193G 661753 2.7.3.3 32.4 - L-arginine pH 7.9, 25°C, mutant enzyme K193G 661753 2.7.3.3 33.4 - ATP isoform AK2 mutant Y89Q, using L-arginine as cosubstrate, in 100 mM Tris/HCl (pH 8.0), at 25°C 722997 2.7.3.3 34.8 - L-Arg mutant enzyme A105S, in 4.76 mM Tris-HCl (pH 8.0), at 25°C 722883 2.7.3.3 35.4 - L-Arg the reaction mixture contains 100 mM Tris/HCl (pH 8), 750 mM KCl, 250 mM Mg-acetate, 25 mM phosphoenolpyruvate made up in 100 mM imidazole/HCl (pH 7), 5 mM NADH made up in Tris/HCl (pH 8), pyruvate kinase/lactate dehydrogenase mixture made up in 100 mM imidazole/HCl (pH 7), 100 mM ATP made up in 100 mM imidazole/HCl (pH 7), and recombinant enzyme, at 25°C 676995 2.7.3.3 36.6 - ATP wild type enzyme isoform AK2, using D-arginine as cosubstrate, in 100 mM Tris/HCl (pH 8.0), at 25°C 722997 2.7.3.3 38.9 - ATP pH 7.5, 25°C, recombinant enzyme 738082 2.7.3.3 38.9 - L-arginine pH 7.5, 25°C, recombinant enzyme 738082 2.7.3.3 39.5 - ATP wild type isoform AK2, using L-arginine as cosubstrate, in 100 mM Tris/HCl (pH 8.0), at 25°C 722997 2.7.3.3 40 - ATP pH 8.0, 25°C, recombinant enzyme 738080 2.7.3.3 40 - L-arginine pH 8.0, 25°C, recombinant enzyme 738080 2.7.3.3 40.8 - ATP pH 7.9, 25°C, mutant enzyme K193R 661753 2.7.3.3 40.8 - D-Arg wild type enzyme isoform AK2, in 100 mM Tris/HCl (pH 8.0), at 25°C 722997 2.7.3.3 40.8 - L-arginine pH 7.9, 25°C, mutant enzyme K193R 661753 2.7.3.3 41 - ATP isoform AK2 mutant G54A, using L-arginine as cosubstrate, in 100 mM Tris/HCl (pH 8.0), at 25°C 722997 2.7.3.3 41.9 - ATP isoform AK2 mutant Y89Q, using D-arginine as cosubstrate, in 100 mM Tris/HCl (pH 8.0), at 25°C 722997 2.7.3.3 41.9 - ATP pH 7.9, 25°C, mutant enzyme N62D 661753 2.7.3.3 41.9 - L-arginine pH 7.9, 25°C, mutant enzyme N62D 661753 2.7.3.3 42.1 - ATP pH 7.9, 25°C, mutant enzyme N62D/K193R 661753 2.7.3.3 42.1 - L-arginine pH 7.9, 25°C, mutant enzyme N62D/K193R 661753 2.7.3.3 43.2 - ATP isoform AK2 mutant G54S, using L-arginine as cosubstrate, in 100 mM Tris/HCl (pH 8.0), at 25°C 722997 2.7.3.3 43.3 - ATP isoform AK2 mutant L64V, using L-arginine as cosubstrate, in 100 mM Tris/HCl (pH 8.0), at 25°C 722997 2.7.3.3 43.3 - L-Arg mutant enzyme A105S/S106G, in 4.76 mM Tris-HCl (pH 8.0), at 25°C 722883 2.7.3.3 44.7 - ATP 25°C, domain 2 642468 2.7.3.3 44.7 - L-Arg 25°C, domain 2 642468 2.7.3.3 45 - ATP pH 8.0, 25°C, recombinant enzyme 738080 2.7.3.3 45 - L-arginine pH 8.0, 25°C, recombinant enzyme 738080 2.7.3.3 45.1 - L-Arg mutant enzyme S106G, in 4.76 mM Tris-HCl (pH 8.0), at 25°C 722883 2.7.3.3 45.9 - L-Arg recombinant wild type enzyme, in 4.76 mM Tris-HCl (pH 8.0), at 25°C 722883 2.7.3.3 46 - L-Arg wild type enzyme isoform AK2, in 100 mM Tris/HCl (pH 8.0), at 25°C 722997 2.7.3.3 47.5 - ATP pH 7.9, 25°C, wild-type enzyme 661753 2.7.3.3 47.5 - L-arginine pH 7.9, 25°C, wild-type enzyme 661753 2.7.3.3 50.1 - ATP isoform AK2 mutant L64I, using L-arginine as cosubstrate, in 100 mM Tris/HCl (pH 8.0), at 25°C 722997 2.7.3.3 50.7 - L-phosphocanavanine - 642451 2.7.3.3 54.16 - ATP pH 8.0, 30°C, recombinant mutant T273G 738395 2.7.3.3 54.16 - L-arginine pH 8.0, 30°C, recombinant mutant T273G 738395 2.7.3.3 56.21 - L-Arg mutant enzyme L113K, in 100 mM Tris, pH 8.0, at 30°C 722403 2.7.3.3 60.3 - ATP pH 8.6, 30°C, mutant enzyme P272R 687093 2.7.3.3 60.36 - L-Arg mutant enzyme I121D, in 100 mM Tris, pH 8.0, at 30°C 722402 2.7.3.3 60.7 - ATP isoform AK2 mutant G54S, using D-arginine as cosubstrate, in 100 mM Tris/HCl (pH 8.0), at 25°C 722997 2.7.3.3 60.97 - ATP pH 8.0, 30°C, recombinant mutant T273A 738395 2.7.3.3 60.97 - L-arginine pH 8.0, 30°C, recombinant mutant T273A 738395 2.7.3.3 61.5 - D-Arg isoform AK2 mutant Y89Q, in 100 mM Tris/HCl (pH 8.0), at 25°C 722997 2.7.3.3 61.8 - ATP isoform AK2 mutant L64V, using D-arginine as cosubstrate, in 100 mM Tris/HCl (pH 8.0), at 25°C 722997 2.7.3.3 62.3 - L-Arg isoform AK2 mutant L64V, in 100 mM Tris/HCl (pH 8.0), at 25°C 722997 2.7.3.3 63.4 - ATP pH 8.0, 25°C, recombinant His6-tagged isozyme AK2 738078 2.7.3.3 63.4 - L-arginine pH 8.0, 25°C, recombinant His6-tagged isozyme AK2 738078 2.7.3.3 64.43 - L-Arg mutant enzyme L113D, in 100 mM Tris, pH 8.0, at 30°C 722403 2.7.3.3 65.8 - ATP isoform AK2 mutant G54A, using D-arginine as cosubstrate, in 100 mM Tris/HCl (pH 8.0), at 25°C 722997 2.7.3.3 68.3 - ATP isoform AK2 mutant L64I, using D-arginine as cosubstrate, in 100 mM Tris/HCl (pH 8.0), at 25°C 722997 2.7.3.3 70.93 - L-Arg mutant enzyme I121K, in 100 mM Tris, pH 8.0, at 30°C 722402 2.7.3.3 73.3 - ATP 25°C, two-domain enzyme 642468 2.7.3.3 73.3 - L-Arg 25°C, two-domain enzyme 642468 2.7.3.3 74.6 - ATP pH 8.6, 30°C, mutant enzyme Y75F/P272G 687093 2.7.3.3 75 - L-arginine pH 8.6, 30°C 762098 2.7.3.3 75.4 - L-Arg isoform AK2 mutant L64I, in 100 mM Tris/HCl (pH 8.0), at 25°C 722997 2.7.3.3 77.8 - L-Arg isoform AK2 mutant Y89Q, in 100 mM Tris/HCl (pH 8.0), at 25°C 722997 2.7.3.3 79.2 - ATP pH 8.6, 30°C, mutant enzyme P272G 687093 2.7.3.3 81 - L-Arg in 100 mM Tris-HCl (pH 8), 750 mM KCl, 250 mM Mg-acetate, 25 mM phosphoenolpyruvate, 5 mM NADH, at 25°C 673493 2.7.3.3 81.2 - L-Arg isoform AK2 mutant G54S, in 100 mM Tris/HCl (pH 8.0), at 25°C 722997 2.7.3.3 84.8 - L-arginine pH 8.0, 20°C 738397 2.7.3.3 86.7 - D-Arg isoform AK2 mutant G54S, in 100 mM Tris/HCl (pH 8.0), at 25°C 722997 2.7.3.3 88 - ATP pH 8.0, 30°C, recombinant wild-type enzyme 738393 2.7.3.3 88 - L-arginine pH 8.0, 25°C, recombinant wild-type enzyme 738393 2.7.3.3 89 - L-Arg mutant two-domain-enzyme D1(Y68G)-D2, in 100 mM Tris/HCl, pH 8.0 at 25°C 722397 2.7.3.3 90.7 - D-Arg isoform AK2 mutant L64I, in 100 mM Tris/HCl (pH 8.0), at 25°C 722997 2.7.3.3 90.7 - D-Arg isoform AK2 mutant L64V, in 100 mM Tris/HCl (pH 8.0), at 25°C 722997 2.7.3.3 104 - ATP pH 8.0, 25°C, recombinant His6-tagged isozyme AK1 738078 2.7.3.3 104 - L-Arg wild type one-domain-enzyme D1, in 100 mM Tris/HCl, pH 8.0 at 25°C 722397 2.7.3.3 104 - L-arginine pH 8.0, 25°C, recombinant His6-tagged isozyme AK1 738078 2.7.3.3 105.6 - L-Arg mutant enzyme L113G, in 100 mM Tris, pH 8.0, at 30°C 722403 2.7.3.3 108 - L-arginine pH 8.0, 15°C, recombinant enzyme 738398 2.7.3.3 109.8 - L-Arg mutant enzyme I121G, in 100 mM Tris, pH 8.0, at 30°C 722402 2.7.3.3 116 - ADP mutant enzyme R312G/E314V/H315D/E317A/E319V 662144 2.7.3.3 116 - N5-(N-phosphonocarbamimidoyl)-L-ornithine mutant enzyme R312G/E314V/H315D/E317A/E319V 662144 2.7.3.3 121 - D-Arg isoform AK2 mutant G54A, in 100 mM Tris/HCl (pH 8.0), at 25°C 722997 2.7.3.3 121 - L-Arg isoform AK2 mutant G54A, in 100 mM Tris/HCl (pH 8.0), at 25°C 722997 2.7.3.3 123 - L-Arg mutant two-domain-enzyme D1-D2(Y68G), in 100 mM Tris/HCl, pH 8.0 at 25°C 722397 2.7.3.3 126.2 - ATP pH 8.6, 30°C, mutant enzyme Y75F 687093 2.7.3.3 129 - ATP recombinant wild-type enzyme with MBP tag. The intact 2D/wild-type enzyme has a higher catalytic constant than the isolated domains 687136 2.7.3.3 129 - L-Arg recombinant wild-type enzyme with MBP tag 687136 2.7.3.3 134 - L-arginine pH 8.0, 17.5°C, recombinant enzyme 738398 2.7.3.3 139 - L-Arg - 642451 2.7.3.3 140 - ADP wild-type enzyme 662144 2.7.3.3 140 - N5-(N-phosphonocarbamimidoyl)-L-ornithine wild-type enzyme 662144 2.7.3.3 140.7 - ATP pH 8.6, 30°C, mutant enzyme Y75D 687093 2.7.3.3 141.3 - ATP pH 8.6, 30°C, mutant enzyme Y75D/P272R 687093 2.7.3.3 143.2 - ATP pH 8.0, 30°C, recombinant mutant T273D 738395 2.7.3.3 143.2 - L-arginine pH 8.0, 30°C, recombinant mutant T273D 738395 2.7.3.3 146.1 - Nomega-phospho-L-arginine pH 7.5, 30°C, arginine kinase TcAK2 760906 2.7.3.3 151.2 - L-Arg mutant enzyme I121L, in 100 mM Tris, pH 8.0, at 30°C 722402 2.7.3.3 151.3 - ATP pH 8.0, 30°C, recombinant mutant T273S 738395 2.7.3.3 151.3 - L-arginine pH 8.0, 30°C, recombinant mutant T273S 738395 2.7.3.3 152.2 - L-Arg mutant enzyme L113I, in 100 mM Tris, pH 8.0, at 30°C 722403 2.7.3.3 157 - L-Arg wild type one-domain-enzyme D2, in 100 mM Tris/HCl, pH 8.0 at 25°C 722397 2.7.3.3 159.4 - ATP - 686213 2.7.3.3 159.4 - ATP pH 8.0, 30°C, recombinant wild-type enzyme 738395 2.7.3.3 159.4 - ATP pH 8.6, 30°C, recombinant arginine kinase 687093 2.7.3.3 159.4 - L-Arg - 673166 2.7.3.3 159.4 - L-Arg recombinant wild type enzyme, in 100 mM Tris, pH 8.0, at 30°C 722402, 722403 2.7.3.3 159.4 - L-arginine - 686213 2.7.3.3 159.4 - L-arginine pH 8.0, 30°C, recombinant wild-type enzyme 738395 2.7.3.3 163 - ATP pH 8.0, 30°C, wild-type enzyme 738395 2.7.3.3 163 - ATP pH 8.6, 30°C, native arginine kinase 687093 2.7.3.3 163 - L-Arg native wild type enzyme, in 100 mM Tris, pH 8.0, at 30°C 722402, 722403 2.7.3.3 163 - L-arginine pH 8.0, 30°C, wild-type enzyme 738395 2.7.3.3 170 - L-arginine pH 8.0, 15°C, recombinant enzyme 738398 2.7.3.3 172.7 - L-arginine pH 7.5, 30°C, arginine kinase TcAK1 760906 2.7.3.3 174 - L-arginine pH 8.0, 20°C, recombinant enzyme 738398 2.7.3.3 180 - ATP pH 8.0, 25°C, recombinant enzyme 738080 2.7.3.3 180 - L-arginine pH 8.0, 25°C, recombinant enzyme 738080 2.7.3.3 183 - L-arginine pH 8.0, 17.5°C, recombinant enzyme 738398 2.7.3.3 187 - L-Arg mutant two-domain-enzyme D1-Lys6-D2, in 100 mM Tris/HCl, pH 8.0 at 25°C 722397 2.7.3.3 196 - L-arginine pH 8.0, 22.5°C, recombinant enzyme 738398 2.7.3.3 212.9 - L-arginine wild-type two-domain enzyme 686224 2.7.3.3 217 - L-arginine pH 8.0, 25°C, recombinant enzyme 738398 2.7.3.3 244 - L-arginine pH 8.0, 20°C, recombinant enzyme 738398 2.7.3.3 272 - L-arginine pH 8.0, 25°C, recombinant enzyme 738398 2.7.3.3 343 - L-arginine pH 8.0, 30°C, recombinant enzyme 738398 2.7.3.3 431 - Nomega-phospho-L-Arg - 642451 2.7.3.3 459 - L-arginine pH 8.0, 35°C, recombinant enzyme 738398 2.7.3.3 678 - L-Arg wild type two-domain-enzyme D1-D2, in 100 mM Tris/HCl, pH 8.0 at 25°C 722397