2.5.1.15 additional information - additional information linear dependency on DHPS concentration when concentration of enzyme versus initial velocity in the coupled enzymatic assay 691070 2.5.1.15 0.0024 - (7,8-dihydropterin-6-yl)methyl diphosphate wild-type enzyme, pH 9.0, 37°C 758651 2.5.1.15 0.0024 - 4-Aminobenzoate wild-type enzyme, pH 9.0, 37°C 758651 2.5.1.15 0.0029 - (7,8-dihydropterin-6-yl)methyl diphosphate mutant enzyme V585A, pH 9.0, 37°C 758651 2.5.1.15 0.0029 - 4-Aminobenzoate mutant enzyme V585A, pH 9.0, 37°C 758651 2.5.1.15 0.0055 - (7,8-dihydropterin-6-yl)methyl diphosphate mutant enzyme A383G, pH 9.0, 37°C 758651 2.5.1.15 0.0055 - 4-Aminobenzoate mutant enzyme A383G, pH 9.0, 37°C 758651 2.5.1.15 0.0057 - 4-Aminobenzoate mutant enzyme A437G/K540E, at pH 9.0 and 37°C 759184 2.5.1.15 0.006 - (7,8-dihydropterin-6-yl)methyl diphosphate mutant enzyme S382A/A383G/A553G, pH 9.0, 37°C 758651 2.5.1.15 0.006 - 4-Aminobenzoate mutant enzyme S382A/A383G/A553G, pH 9.0, 37°C 758651 2.5.1.15 0.0075 - 4-Aminobenzoate mutant enzyme S436A/A437G, at pH 9.0 and 37°C 759184 2.5.1.15 0.0077 - 4-Aminobenzoate mutant enzyme S436F/A437G/A613S, at pH 9.0 and 37°C 759184 2.5.1.15 0.01 - 4-Aminobenzoate mutant enzyme A613T, at pH 9.0 and 37°C 759184 2.5.1.15 0.01 - 4-Aminobenzoate mutant enzyme S436F/A613T, at pH 9.0 and 37°C 759184 2.5.1.15 0.011 - 4-Aminobenzoate mutant enzyme K540E, at pH 9.0 and 37°C 759184 2.5.1.15 0.012 - 4-Aminobenzoate mutant enzyme A613S, at pH 9.0 and 37°C 759184 2.5.1.15 0.0134 - (7,8-dihydropterin-6-yl)methyl diphosphate mutant enzyme A383G/A553G, pH 9.0, 37°C 758651 2.5.1.15 0.0134 - 4-Aminobenzoate mutant enzyme A383G/A553G, pH 9.0, 37°C 758651 2.5.1.15 0.0137 - 4-Aminobenzoate mutant enzyme S436F/A437G/A613T, at pH 9.0 and 37°C 759184 2.5.1.15 0.014 - 4-Aminobenzoate mutant enzyme S436F/A613S, at pH 9.0 and 37°C 759184 2.5.1.15 0.015 - 4-Aminobenzoate wild type enzyme, at pH 9.0 and 37°C 759184 2.5.1.15 0.02 - 4-Aminobenzoate mutant enzyme A437G, at pH 9.0 and 37°C 759184 2.5.1.15 0.029 - 4-Aminobenzoate mutant enzyme A581G, at pH 9.0 and 37°C 759184 2.5.1.15 0.032 - (2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate mutant DELTA74-80 705600 2.5.1.15 0.035 - (2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate mutant DELTA257-306 705600 2.5.1.15 0.0578 - dapson in absence of 4-aminobenzoate 691070 2.5.1.15 0.068 - (2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate mutant DELTA247-306 705600 2.5.1.15 0.069 - (2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate wild type 705600 2.5.1.15 0.079 - sulfamethizole in absence of 4-aminobenzoate 691070 2.5.1.15 0.08 - sulfanilamide in absence of 4-aminobenzoate 691070 2.5.1.15 0.0887 - sulfathiazole in absence of 4-aminobenzoate 691070 2.5.1.15 0.109 - 4-Aminobenzoate 100 microM DHP-PP, 25 °C 691070 2.5.1.15 0.11 - sulfisoxazole in absence of 4-aminobenzoate 691070 2.5.1.15 0.12 - sulfamethoxazole in absence of 4-aminobenzoate 691070 2.5.1.15 0.1243 - Sulfadiazine in absence of 4-aminobenzoate 691070