1.8.4.9 0.00075 - 3'-phosphoadenosine 5'-phosphosulfate - 687585 1.8.4.9 0.012 - 3'-phosphoadenosine-5'-phosphosulfate assay conditions: APS reductase (0.0009 mM), EST (0.10 unit/ml), thioredoxin (0.037 mM), MgCl2 (3.0 mM), DTT (2.0 mM), pNPS (10 mM), GSH (5.0 mM), and Hepes (50 mM, pH 8.0), at 25°C 667703 1.8.4.9 0.153 - 5'-adenylylsulfate assay conditions: APS reductase (0.00021 mM), PK (8.4 units/ml), LDH (18.0 units/ml), adenylate kinase (10 units/ml), thioredoxin (0.0373 mM), MgCl2 (3.0 mM), DTT (2.0 mM), ATP (1.0 mM), PEP (1.0 mM), EDTA (1.0 mM), GSH (5.0 mM), NADH (0.030 mM), and Hepes (50 mM, pH 8.0), at 25°C 667703 1.8.4.9 0.176 - adenosine 5'-phosphosulfate - 687585 1.8.4.9 2.1 - 5'-adenylylsulfate with glutathione as reductant 288642 1.8.4.9 3.4 - 5'-adenylylsulfate with DTT as reductant 288642 1.8.4.9 8.4 - 5'-adenylyl sulfate 30°C, pH 8.5, cosubstrate: dithiothreitol 689431 1.8.4.9 8.4 - glutathione 30°C, pH 8.5 689431 1.8.4.9 10.1 - dithiothreitol 30°C, pH 8.5 689431 1.8.4.9 11.3 - 5'-adenylyl sulfate 30°C, pH 8.5, cosubstrate: glutathione 689431 1.8.4.9 56 - 5'-adenylylsulfate - 288644